大肠癌组织和细胞中KDM3A的表达及其对癌细胞增殖、侵袭和迁移的影响  

Expression of KDM3A in tissues and cells of colorectal cancer and its effect on proliferation,invasion and migration of cancer cells

作　　者：罗志鹏 苗志国[1] 魏海云[1] LUO Zhipeng;MIAO Zhiguo;WEI Haiyun(Department of Digestive Tumor,Jiangxi Cancer Hospital,Jiangxi Nanchang 330029,China)

机构地区：[1]江西省肿瘤医院腹部肿瘤外科,江西南昌330029

出　　处：《现代肿瘤医学》2023年第13期2395-2400,共6页Journal of Modern Oncology

基　　金：江西省卫生健康委科技计划(编号:20201093)。

摘　　要：目的:研究大肠癌组织和细胞中赖氨酸组蛋白甲基化酶(lysine histone demethylase 3A,KDM3A)的表达及其对大肠癌细胞增殖、迁移和侵袭的影响。方法:通过生物信息学检索分析公开数据库中KDM3A在大肠癌组织中的表达及相关临床信息。采用qRT-PCR技术检验KDM3A在4种大肠癌细胞HT-29、SW480、SW620、DLD-1以及正常人结肠上皮细胞NCM460中的表达水平,筛选出KDM3A表达量低的大肠癌细胞系进行过表达并进行CCK-8增殖实验、Transwell实验及Western blot检测KDM3A过表达对细胞增殖、侵袭及迁移的影响。结果:KDM3A在大肠癌组织中表达明显高于癌旁组织,且KDM3A高表达患者生存率更低。过表达KDM3A可以促进大肠癌细胞的增殖、迁移和侵袭能力。结论:高表达的KDM3A可以促进大肠癌细胞的增殖、迁移和侵袭。Objective:To study the expression of lysine histone methylase 3A(KDM3A)in tissues and cells of colorectal cancer and its effect on the proliferation,migration and invasion of colorectal cancer cells.Methods:The expression and clinical information of KDM3A in colorectal cancer tissues were analyzed by bioinformatics retrieval.qRT-PCR was used to detect the expression levels of KDM3A in 4 colorectal cancer cells HT-29,SW480,SW620,DLD-1 and normal colon epithelial cells NCM460.Colorectal cancer cell lines with low KDM3A expression were screened out for overexpression and CCK-8 proliferation assay and Transwell assay.Western blot was used to detect the effect of KDM3A overexpression on cell proliferation,cell invasion and migration.Results:The expression of KDM3A in colorectal cancer tissues is significantly higher than that in adjacent tissues,and the survival rate of patients with high expression of KDM3A is lower.Overexpression of KDM3A can promote the proliferation,migration and invasion of colorectal cancer cells.Conclusion:High expression of KDM3A can promote the proliferation,migration and invasion of colorectal cancer.

关 键 词：KDM3A 大肠癌 增殖 侵袭 迁移 

分 类 号：R735.3[医药卫生—肿瘤]