C-MYC/BCL2双表达弥漫大B细胞淋巴瘤组织中PIM1的表达及与临床病理特征的关系  

The expression of PIM1 in diffuse large B-cell lymphoma tissue with co-expression of C-MYC/BCL2 and its relationship with clinicopathological characteristics

作　　者：徐睿欢 许娟 杨文秀 XU Ruihuan;XU Juan;YANG Wenxiu(Department of Pathology,the Affiliated Hospital of Guizhou Medical University,Guizhou Guiyang 550001,China)

机构地区：[1]贵州医科大学附属医院病理科,贵州贵阳550001

出　　处：《现代肿瘤医学》2023年第13期2491-2496,共6页Journal of Modern Oncology

基　　金：贵州省贵阳市科技计划项目[编号:筑科合同(2019)9-1-6号]。

摘　　要：目的:检测C-MYC/BCL2双表达弥漫大B细胞淋巴瘤(DEL)组织中莫罗尼小鼠白血病病毒前病毒整合位点(PIM)1基因突变及表达情况,探讨PIM1表达与DEL临床病理特征的关系。方法:收集2010年01月01日至2018年12月31日我院病理科确诊的弥漫大B细胞淋巴瘤(DLBCL)临床病例,采用免疫组织化学染色检测C-MYC和BCL2蛋白表达,从中筛选出合格病例进行研究,检测p65表达;sanger测序检测PIM1突变,RT-qPCR检测PIM1 mRNA的表达并进行统计学分析。结果:DEL中PIM1基因突变及其对PIM1表达的影响:纳入研究的39例DEL中PIM1的突变率为56.41%(22/39)。PIM1 mRNA在PIM1基因突变组与未突变组中表达有统计学差异(P=0.012),突变组病例明显升高。NF-κB(p65)蛋白表达:p65核阳性表达率53.85%(21/39),non-GCB型(16/23)病例的阳性表达率显著高于GCB型(5/16)DEL(P=0.025)。PIM1表达与DEL临床病理特征的关系:PIM1 mRNA的表达与Ann Arbor分期、血清LDH水平有关,在晚临床分期和血清LDH较高的DEL病例中明显上升(P=0.046,P=0.048)。PIM1、p65对DEL中C-MYC及BCL2表达的影响:p65阳性与阴性表达组之间C-MYC蛋白表达程度有统计学差异(P=0.021),阳性表达组C-MYC表达明显增高。DEL中C-MYC和BCL2表达与PIM1 mRNA表达有关,高表达者PIM1 mRNA表达明显上调(P分别为0.015和0.039)。结论:DEL中存在PIM1基因的高频突变,PIM1 mRNA的上调与其突变有关,PIM1异常可能与DEL的不良预后特征有关;p65影响DEL中C-MYC蛋白表达,但与PIM1异常无关。Objective:To detect the mutation and expression of PIM1 gene in C-MYC/BCL2 double-expression diffuse large B-cell lymphoma(DEL),and to explore the relationship between PIM1 expression and clinical pathological features of DEL.Methods:Clinical cases diagnosed as DLBCL by the Department of Pathology of our hospital from January 1,2010 to December 31,2018 were collected.Immunohistochemistry(IHC)staining was used to detect the expression of C-MYC and BCL2 proteins.Qualified cases were selected for detecting the expression of p65.sanger sequencing were used to detect PIM1 mutations,and RT-qPCR was used to measure the expression of PIM1 mRNA.Cases were followed up and appropriate statistical methods were used for statistical analysis.Results:PIM1 gene mutation in DEL cases and its effect on PIM1 expression:39 DEL cases were included,the total mutation rate of PIM1 in DEL was 56.41%(22/39).The expression of PIM1 mRNA in the cases with/without PIM1 mutation was statistically different(P=0.012),and it was significantly increased in the PIM1 mutation group.NF-κB(p65)protein expression:The total positive expression rate was 53.85%(21/39),and respectively in non-GCB type DEL(16/23)was significantly higher than that in GCB type DEL(5/16)(P=0.025).The relationship between PIM1 expression and clinicopathological characteristics of DEL:The expression of PIM1 mRNA was also related to Ann Arbor staging and serum LDH level.It is significantly increased in late clinical stage or DEL cases with abnormally elevated serum LDH(P=0.046,P=0.048).The effect of PIM1,p65 on the expression of C-MYC and BCL2 in DEL:The expression of C-MYC protein in the p65 positive was statistically significantly increased compared with negative expression group(P=0.021).In DEL cases,the expression of C-MYC and BCL2 has relationship with PIM1 mRNA,PIM1 mRNA significantly increased with high expression(P=0.015,0.039).Conclusion:In DEL,there are high-frequency mutations in the PIM1 gene and up-regulation of RNA expression,the up-regulation of RNA expression of

关 键 词：双表达淋巴瘤 弥漫大B细胞淋巴瘤 Hans分型 PIM1 P65 预后 

分 类 号：R733.4[医药卫生—肿瘤]