Astragalus adscendens extract shows antidiabetic effects through controlling oxidative stress,inflammation and apoptosis in streptozotocin-induced diabetic rats  

作　　者：Yosra Raziani Kimia Karami Hamid Reza Mohammadi Hossein Mahmoudvand Mohammad Nabi Moradi Javad Ghasemian Yadegari 

机构地区：[1]Nursing Department,Al-Mustaqbal University College,51001 Hillah,Babylon,Iraq [2]Razi Herbal Medicines Research Center,Lorestan University of Medical Sciences,Khorramabad,Iran [3]Molecular and Cellular Laboratory,School of Allied Medicine,Lorestan University of Medical Sciences,Khorramabad,Iran

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2023年第6期242-249,共8页亚太热带生物医学杂志（英文版）

摘　　要：Objective:To assess the effect of oral treatment of methanolic extract of the aerial parts of Astragalus adscendens in streptozotocin-induced diabetic rats.Methods:In order to induce diabetes,rats intraperitoneally received streptozotocin at 65 mg/kg.Sixty adult male Wistar rats were allocated into six groups(10 rats per each)including the healthy control group,the diabetic group as well as the diabetic group treated with Astragalus adscendens methanolic extract at 50,100,and 200 mg/kg per day or glibenclamide(0.6 mg/kg/day)for 28 d.The effects of Astragalus adscendens methanolic extract on the levels of glucose,insulin,alanine aminotransferase,alkaline phosphatase,aspartate aminotransferase,bilirubin,creatinine,urea,uric acid,total protein,albumin,triglyceride,cholesterol,α-amylase,oxidant/antioxidant enzymes,and inflammatory cytokines were evaluated.Real time-PCR was also used for measuring the gene expression of caspase-3,Bcl2,and Bax.Results:The levels of glucose,cholesterol,triglyceride,creatinine,urea,uric acid,alanine aminotransferase,aspartate aminotransferase,alkaline phosphatase,bilirubin,and malondialdehyde considerably declined(P<0.001)in diabetic rats after treatment with Astragalus adscendens methanolic extract especially at a dose of 200 mg/kg.In addition,treatment with Astragalus adscendens methanolic extract noticeably increased the level of insulin,total protein,and albumin as well as improved the activities of catalase,glutathione peroxidase,and superoxide dismutase,as well as the expression levels of TNF-α,IL-1β,caspase-3,Bcl2 and Bax(P<0.001)compared to the diabetic control group.The extract also inhibitedα-amylase in a dose-dependent manner with an IC_(50)value of 19.6μg/mL.Conclusions:Astragalus adscendens methanolic extract shows potent antidiabetic,anti-inflammatory,anti-apoptotic,and antioxidant effects in diabetic rats.However,more studies are needed to verify the underlying mechanism of the effect of this plant extract and test its efficacy in clinical trials.

关 键 词：Herbal medicines Astragalus adscendens Diabetes ANTIOXIDANT Antidiabetes STREPTOZOTOCIN ANTIINFLAMMATION 

分 类 号：R285.5[医药卫生—中药学]