GC-FID快速测定人粪便中7种短链脂肪酸含量的方法研究  

Study on rapid determination of short-chain fatty acids in human feces by GC-FID

作　　者：张炎[1] 张晓旭[2] 甄少波 张琪[2] 郭兆斌[1] ZHANG Yan;ZHANG Xiaoxu;ZHEN Shaobo;ZHANG Qi;GUO Zhaobin(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China;Department of Nutrition and Health,China Agricultural University,Beijing 100190,China;School of Hotel Management,China University of Labor Relations,Beijing 100048,China)

机构地区：[1]甘肃农业大学食品科学与工程学院,甘肃兰州730070 [2]中国农业大学营养与健康系,北京100190 [3]中国劳动关系学院酒店管理学院,北京100048

出　　处：《实验技术与管理》2023年第5期82-88,共7页Experimental Technology and Management

基　　金：甘肃农业大学科技创新基金-青年导师扶持基金资助项目(0522205);甘肃省自然科学基金项目(20JR10RA523)。

摘　　要：短链脂肪酸是结肠粘膜的重要能量来源,具有协调肠道菌群稳态、调节免疫及抗炎等功能。为准确、快速表征机体健康状况及体内肠道菌群丰度,该文建立了一种成本低、灵敏度高、检测速度快的人粪便中短链脂肪酸气相色谱检测方法。结果表明,取10 mg 4℃保存的新鲜样本,先用50%(w/w)的浓硫酸酸解,再用乙醚提取2次,乙酸、丙酸、丁酸、异丁酸、戊酸、异戊酸、己酸的提取率均超过97%;GC-FID法检测短链脂肪酸的最低检测限为0.01~0.02 mM,最低定量限为0.02~0.08 mM,加标回收率为83.32%~111.89%,日内精密度为0.91%~1.79%,日间精密度为1.21%~2.29%。健康人群及功能性消化不良患者粪便样本实验结果表明,该方法准确度优、操作简单、成本低,能够为大样本量人群实验提供指导。Short-chain fatty acids are important energy sources exisiting in colon mucosa,which have functions of coordinating intestinal flora homeostasis,regulating immunity and anti-inflammation.In order to accurately and rapidly ascertain whether the body is healthy and abundance of intestinal flora,a low-cost and high sensitivity detection method for short-chain fatty acids in human feces by gas chromatography was established.The results showed that the extraction rate of acetic acid,propionic acid,butyric acid,isobutyric acid,valeric acid,isovaleric acid and caproic acid was more than 97%in 10 mg fresh samples stored at 4℃.The lowest detection limits of short-chain fatty acids by GC-FID were 0.01~0.02 mM,and the lowest limits of quantitation were 0.02~0.08 mM.The standard recoveries were 83.32%~111.89%,with intra-day precision of 0.91%~1.79%and intra-day precision of 1.21%~2.29%.The experimental results of fecal samples from healthy people and patients with functional dyspepsia showed that the method had excellent accuracy,simple operation and low cost.The experimental results provide guidance for large sample crowd experiments.

关 键 词：短链脂肪酸 气相色谱 人群实验 肠道菌群 

分 类 号：TS07[轻工技术与工程]