人参皂苷Rg1与栀子苷配伍对PS1 D385A小鼠脑血管Aβ水平的影响研究  

Effect of Ginsenoside Rgl and Gardenoside on Cerebral Vascular Aβof PS1 D385A Mice Study on the Impact of Level

作　　者：陈伟航 华茜 张华伟 彭甜甜 刘小鸽 邓晨耕 司展华 常珂馨 王旭 谭琰 CHEN Wei-hang;HUA Qian;ZHANG Hua-wei;PENG Tian-tian;LIU Xiao-ge;DENG Chen-geng;SI Zhan-hua;CHANG Ke-xin;WANG Xu;TAN Yan(School of Acupuncture and Massage,Beijing University of Traditional Chinese Medicine,Beijing,100029,China;School of Life Sciences,Beijing University of Traditional Chinese Medicine,Beijing,100029,China;School of Chinese Medicine,Beijing University of Traditional Chinese Medicine,Beijing,100029,China)

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]北京中医药大学生命科学学院,北京100029 [3]北京中医药大学中医学院,北京100029

出　　处：《现代生物医学进展》2023年第11期2001-2007,共7页Progress in Modern Biomedicine

基　　金：国家自然科学基金重点项目(U21A20414)。

摘　　要：目的:基于PS1 D385A基因敲进模型小鼠,观察人参皂苷Rg1(Ginsenoside Rg1)与栀子苷(Geniposide, GP)配伍对脑血管内淀粉样斑块清除和脑血管生成的影响,探讨其影响对阿尔兹海默症(Alzheimer’s Disease, AD)治疗的意义。方法:以PS1 D385A KI/+小鼠为模型,对PS1 D385A KI/+小鼠进行Rg1和GP灌胃给药,并以同窝健康小鼠作为对照,采用免疫荧光技术和Western blot技术,检测各组小鼠大脑皮层及海马区血管内淀粉斑块清除和血管生成水平。结果:Rg1+GP组皮质区和海马区CD31的表达量显著降低;Rg1+GP组皮质顶叶颞叶区和海马齿状回区CD31标记的血管网信号密度明显降低;与KI/+组小鼠相比,Rg1+GP组小鼠海马区血管内Aβ40沉积水平显著降低。结论:Rg1与GP配伍可以减少PS1 D385A KI/+成年小鼠皮质顶叶颞叶区和海马齿状回区的血管生成异常的情况。Objective:Based on the PS1 D385A gene knock-in model mice,to observe the effect of Rg1 and GP compatibility on the clearance of amyloid plaque in cerebral blood vessels and cerebral angiogenesis,and to explore the significance of its effect on the treatment of AD(Alzheimer's disease).Methods:Taking PS1 D385A KI/+mice as the model,the PS1 D385A KI/+mice were given Rgl and GP by gavage,and the healthy mice in the same litter as the control.The levels of starch plaque clearance and angiogenesis in the cerebral cortex and hippocampus of mice in each group were measured by immunofluorescence and Western blot.Results:The expression of CD31 in cortex and hippocampus of Rgl+GP group was significantly decreased;In Rg1+GP group,the signal density of CD31 labeled vascular network in the temporal lobe of the parietal lobe and the dentate gyrus of the hippocampus decreased significantly;Compared with KI/+group mice,the level of Aβ40 deposition in the hippocampus of Rg1+GP group mice was significantly decreased.Conclusion:The combination of Rgl and GP can reduce the abnormal angiogenesis in the temporal lobe of the parietal cortex and the dentate gyrus of the hippocampus ofPS1D385AKI/+adult mice.

关 键 词：早老素基因 阿尔兹海默症 人参皂苷RG1 栀子苷 淀粉样斑块β 

分 类 号：R-33[医药卫生] Q593.2[生物学—生物化学] R243