罗哌卡因调控PI3K/Akt信号通路对肝癌细胞功能的作用研究  

Effects of Ropivacaine on Hepatocellular Carcinoma Cell Function by Regulating PI3K/Akt Signaling Pathway

作　　者：罗倩 杨颖[1] 付庆江[1] 曹立瀛[1] 李振华[1] Luo Qian;Yang Ying;Fu Qingjiang(Hepatobiliary Surgery,Kailuan General Hospital,Hepatobiliary Surgery with Integrated Traditional Chinese and Western Medicine,Kailuan General Hospital,Tangshan,Hebei 063000,China)

机构地区：[1]开滦总医院肝胆外科,中西医结合肝胆外科,河北唐山063000

出　　处：《四川医学》2023年第5期471-477,共7页Sichuan Medical Journal

摘　　要：目的 探究罗哌卡因对人肝癌细胞黏附、迁移、侵袭和上皮间质转化(EMT)的影响及对磷脂酰肌醇-3-激酶/蛋白激酶B(PI3K/Akt)信号通路的调控作用。方法 体外培养人肝癌MHCC97H细胞,分为对照组(不做干预),实验组(200、400、600和800μmol/L罗哌卡因)和抑制剂组(200μmol/L罗哌卡因+30μmol/L PI3K/Akt通路抑制剂LY294002),干预24 h。用细胞计数试剂盒(CCK-8)法测定细胞活力,采用细胞黏附实验测定黏附细胞数,用Transwell小室测定细胞的迁移和侵袭能力,实时荧光定量PCR(RT-qPCR)及蛋白免疫印迹(WB)法对细胞EMT和PI3K/Akt通路相关因子表达水平进行分析。结果 与对照组比较,800μmol/L罗哌卡因的细胞活力降低(P<0.05);200、400和600μmol/L罗哌卡因的细胞黏附数、迁移数、侵袭数以及p-PI3K、p-Akt、N-钙黏蛋白(N-cadherin)和纤连蛋白(FN)表达量减少(P<0.05),E-钙黏蛋白(E-cadherin)mRNA和蛋白表达量增加(P<0.05);与200μmol/L罗哌卡因组相比,抑制剂组上述指标变化更为显著(P<0.05)。结论 罗哌卡因可能是通过抑制PI3K/Akt信号通路来抑制人肝癌MHCC97H细胞的黏附、迁移、侵袭和EMT进程。Objective To study effects of ropivacaine on the adhesion,migration,invasion and epithelial-mesenchymal transition(EMT)of human hepatocellular carcinoma cells and the regulation of phosphatidylinositol-3-kinase/protein kinase B(PI3K/Akt)signaling pathway.Methods In vitro experiments,human hepatoma MHCC97H cells were divided into control group(without intervention),experimental group(200,400,600 and 800μmol/L ropivacaine)and inhibitor group(200μmol/L ropivacaine+30μmol/L PI3K/Akt signaling pathway inhibitor LY294002),intervened for 24 h.Cell viability was determined by the cell counting kit-8(CCK-8),cell adhesion assay was used to determine the number of adherent cells,the migration and invasion of cells were determined by Transwell assay,EMT and PI3K/Akt pathway-related factors were analyzed by real-time quantitative PCR(RT-qPCR)and western blotting(WB).Results Compared with control group,the cell viability at 800μmol/L ropivacaine was decreased(P<0.05).The number of cell adhesion,migration,invasion and p-PI3K,p-Akt,N-cadherin and fibronectin(FN)were decreased,while E-cadherin mRNA and protein were increased at 200,400 and 600μmol/L ropivacaine(P<0.05).Compared with 200μmol/L ropivacaine group,the above indexes in inhibitor group were more significantly changed(P<0.05).Conclusion Ropivacaine inhibit adhesion,migration,invasion and EMT process of human hepatoma MHCC97H cells,possibly by inhibiting the PI3K/Akt signaling pathway.

关 键 词：罗哌卡因 肝癌 磷脂酰肌醇-3-激酶/蛋白激酶B信号通路 黏附 迁移 侵袭 上皮间质转化 

分 类 号：R735.7[医药卫生—肿瘤]