PDGFRA调控p53信号通路介导卵巢癌细胞增殖、凋亡和侵袭的作用及机制  

Role and mechanism of PDGFRA in regulating p53 signaling pathway to mediate proliferation,apoptosis and invasion of ovarian cancer cells

作　　者：熊廷川 王迎洪[2] 朱长军[3] XIONG Tingchuan;WANG Yinghong;ZHU Changjun(Department of Gynecologic Surgery,The 3rd Affiliated Teaching Hospital of Xinjiang Medical University(Affiliated Cancer Hospital),Urumqi 830011,China;Center of Heath Management,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,China;Tianjin Key Laboratory of Animal and Plant Resistance,Tianjin Normal University,Tianjin 300387,China)

机构地区：[1]新疆医科大学第三临床医学院(附属肿瘤医院),妇外三科,乌鲁木齐830011 [2]新疆医科大学第一附属医院健康管理中心,乌鲁木齐830011 [3]天津师范大学天津市动植物抗性重点实验室,天津300387

出　　处：《天津师范大学学报（自然科学版）》2023年第3期35-39,52,共6页Journal of Tianjin Normal University：Natural Science Edition

基　　金：天津市多元投入基金重点资助项目(21JCZDJC00960);新疆维吾尔自治区科技支疆项目(2017E0263);新疆医科大学科研创新基金资助项目(XYDCX201569)。

摘　　要：为了探究血小板源性生长因子受体α(platelet derived growth factor receptorα,PDGFRA)调控卵巢癌细胞的增殖、凋亡和侵袭的作用以及具体机制,首先基于GEO数据库中相关基因芯片(GSE4122和GSE14407)分析验证PDGFRA在卵巢癌中存在的表达差异,再应用SangerBox在线分析PDGFRA与卵巢癌患者预后的关联性.构建PDGFRA过表达质粒,用MTT检测PDGFRA对OVCAR3细胞增殖的影响,用流式细胞术检测PDGFRA对OVCAR3细胞凋亡的影响,用Transwell检测PDGFRA对OVCAR3细胞侵袭能力的影响,用Western blot法检测p-p53、p53及p21蛋白的表达水平.结果发现:PDGFRA在卵巢癌中显著上调,与PDGFRA低表达患者相比,PDGFRA高表达患者的预后更差;与vector-NC相比,PAGFRA组细胞的增殖和侵袭显著提高,细胞凋亡及p-p53、p21蛋白的表达水平均显著下降.由此得出,PDGFRA通过介导p53信号通路调控影响OVCAR3细胞的增殖、凋亡和侵袭.To investigate the role and specific mechanism of platelet derived growth factor receptorα(PDGFRA)in regulat-ing the proliferation,apoptosis and invasion of ovarian cancer cells,differential expression of PDGFRA in ovarian cancer was verified firstly based on the analysis of relevant gene chips(GSE4122 and GSE14407)in the GEO database.Then SangerBox was used to analyse the association between PDGFRA and the prognosis of ovarian cancer patients online.The PDGFRA over-expression plasmid was created,the effect of PDGFRA on OVCAR3 cell proliferation was explored using MTT,the effect of PDGFRA on OVCAR3 cell apoptosis was detected using flow cytometry,the effect of PDGFRA on OVCAR3 cell invasion was detected using Transwell,and the expression levels of p-p53,p53 and p21 proteins were measured using Western blot.The results showed that PDGFRA expression was significantly up-regulated in ovarian cancer,and patients with high PDGFRA expression had a worse prognosis compared to those with low PDGFRA expression.Cell proliferation and invasion were signifi-cantly higher in the PAGFRA group compared to vector-NC group,while apoptosis and p-p53 and p21 protein expression levels were significantly lower.It is concluded that PDGFRA can influence OVCAR3 cell proliferation,apoptosis and invasion by me-diating the regulation of the p53 signalling pathway.

关 键 词：卵巢癌 差异表达基因 血小板源性生长因子受体α p53信号通路 

分 类 号：Q291[生物学—细胞生物学] R737.31[医药卫生—肿瘤]