三种桑黄子实体脂溶性成分的GC-MS分析及抗氧化活性  

GC-MS Analysis and Antioxidant Activities of Liposoluble Components from Three Sanghuangporus Fruit Bodies

作　　者：罗阳兰 曹乃馨 杨玉梅 黄丽玲 阎勇[1] 黄世旅[1] LUO Yang-lan;CAO Nai-xin;YANG Yu-mei;HUANG Li-ling;YAN Yong;HUANG Shi-Lv(Microbiology Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007;Shaanxi University of Technology,Hanzhong 723000;Lueyang County Monitoring and Testing Center of Agricultural Products Quality Safety,Hanzhong 724300)

机构地区：[1]广西农业科学院微生物研究所,南宁530007 [2]陕西理工大学,汉中723000 [3]略阳县农产品质量安全监测检验中心,汉中724300

出　　处：《生物技术通报》2023年第6期149-157,共9页Biotechnology Bulletin

基　　金：广西农业科学院科技发展基金项目(桂农科2021JM85);广西农业科学院科技发展基金项目(桂农科2022JM07)。

摘　　要：比较分析不同桑黄子实体脂溶性成分差异,并初步探究其抗氧化活性。采用索氏提取法获得脂溶性成分并进行GC-MS分析,计算各化学成分的相对百分含量,测定其还原能力、对DPPH自由基、羟自由基的清除能力分析抗氧化活性。GC-MS结果表明共鉴定到176种化合物,JM1、JM2和JM3的脂溶性成分分别有68、75和71种化合物,对化合物进行归类分析发现主要含有烷类、酯类、芳香族类等物质,共有成分共8种,相对含量分别占JM1、JM2和JM3提取物的12.88%、9.85%、9.54%,分别为2,4-二甲基庚烷、乙苯、对二甲苯、苯乙烯、6-十二酮、棕榈酸甲酯、木蜡酸甲酯和四十四烷。3种子实体中JM1脂溶性物质具有更好的抗氧化活性。桑黄子实体脂溶性成分含有多种活性物质,具有一定的生物活性,可为深入研究其药理功效和资源开发提供参考。This work aims to study components and antioxidant activities of liposoluble components from different Sanghuangporus sp.fruiting bodies.Soxhlet extraction method was applied to extract the liposoluble components from different Sanghuangporus sp.fruiting bodies and their chemical compositions were identified by GC-MS.Its reducing ability was determined,and the antioxidant activity was evaluated by the reducing capacity,1,1-diphenyl 1-diphenyl-2-picrylhydrazyl(DPPH)free radical scavenging ability and hydroxyl free radical scavenging ability.The GC-MS results suggested that there were 176 identified liposoluble components in total.Liposoluble components of JM1,JM2 and JM3 were 68,75,71 compounds,respectively.After classification and analysis,the compounds mainly contained alkane,ester,aromatic group and other substances.There are 8 common components,and their relative contents account for 12.88%,9.85%and 9.54%of JM1,JM2 and JM3 extracts,respectively,which were 2,4-dimethylheptane,ethylbenzene,1,4-xylene,styrene,6-dodecanone,methyl hexadecanoate,methyl tetracosanoate and tetratetracontane.JM1 liposoluble components had the best antioxidant activities.The liposoluble components of three Sanghuangporus sp.fruiting bodies contained a variety of active substances and had various biological activities,which can provide a reference for the further study of their pharmacological efficacy and resource development.

关 键 词：桑黄 脂溶性成分 气相色谱质谱联用仪 抗氧化活性 

分 类 号：R284.1[医药卫生—中药学]