外泌体lncRNA-MIR100HG/miR-100表达变化与胃癌临床病理特征、无疾病进展生存率的相关性  

Correlation between the Expression of LncRNA-MIR100HG/miR-100 in Exosomes and Clinicopathological Characteristics and Progression Free Survival Rate of Gastric Cancer

作　　者：王晓丽 王燕[1] 宋诸臣[1] 肖金章 宋佳烨 曹永峰[1] WANG Xiao-li;WANG Yan;SONG Zhu-chen;XIAO Jin-zhang;SONG Jia-ye;CAO Yong-feng(Department of Oncology,Tumor Hospital Affiliated to Nantong University,Nantong,Jiangsu,226361,China)

机构地区：[1]南通大学附属肿瘤医院肿瘤内科,江苏南通226361

出　　处：《现代生物医学进展》2023年第12期2305-2309,2357,共6页Progress in Modern Biomedicine

基　　金：江苏省基础研究计划(自然科学基金)——面上项目(BK20191208);南通市卫生健康委员会科研课题(MA2020008)。

摘　　要：目的:探究胃癌患者血清外泌体长链非编码RNA-MIR100HG(lncRNA-MIR100HG)及微小核糖核酸-100(miR-100)表达情况,并分析其与患者临床病理特征和无疾病进展生存率之间的相关性。方法:收集60例胃癌患者和60例良性疾病患者,提取外泌体,检测lncRNA-MIR100HG/miR-100的表达情况并进行组间比较。采用Pearson相关分析lncRNA-MIR100HG与miR-100表达水平的相关性,应用单因素卡方检验分析与胃癌患者临床病理特征相关性,采用Kaplan-Meier生存分析lncRNA-MIR100HG/miR-100表达情况与无疾病进展生存率。结果:(1)胃癌组患者血清lncRNA-MIR100HG显著高于,miR-100相对表达水平显著低于胃良性疾病组(P<0.05);(2)Pearson相关分析结果显示血清lncRNA-MIR100HG和miR-100存在显著负相关关系(r=-0.483,P<0.05);(3)单因素卡方检验分析结果显示:胃癌患者血清lncRNA-MIR100HG相对表达水平与肿瘤大小、分化程度、肿瘤浸润深度、临床分期、淋巴结转移和远处转移相关,血清miR-100相对表达水平与肿瘤大小、分化程度、临床分期和淋巴结转移相关(P<0.05);(4)血清lncRNA-MIR100HG、miR-100低水平表达胃癌患者PFS显著长于高水平患者(χ^(2)=37.371,P<0.05),miR-100高水平表达胃癌患者PFS显著长于低水平患者(χ^(2)=28.631,P<0.05)。结论:胃癌患者血清外泌体lncRNA-MIR100HG/miR-100表达水平与肿瘤大小、肿瘤浸润深度、临床分期等病理特征相关,lncRNA-MIR100HG高表达与miR-100低表达可能提示胃癌患者预后越差。Objective:To investigate the expression of long chain non coding RNA-MIR100HG(lncRNA-MIR100HG)and microribonucleic acid-100(miR-100)in serum exosomes of patients with gastric cancer,and analyze the correlation between them and clinical pathological characteristics and progression free survival rate.Methods:Blood samples from 60 patients with gastric cancer and 60 patients with benign diseases were collected,and the exosomes were extracted.The expression of lncRNA-MIR100HG/miR-100 was detected and compared between groups.Pearson correlation was used to analyze the correlation between the expression level of lncRNA-MIR100HG and miR-100,single factor chi square test was used to analyze the correlation with the clinicopathological characteristics of gastric cancer patients,and Kaplan-Meier survival was used to analyze the expression of lncRNA-MIR100HG/miR-100 and the progression free survival rate.Results:(1)The level of serum lncRNA-MIR100HG in patients with gastric cancer was higher than that in patients with gastric cancer,and the relative expression level of miR-100 was lower than that in patients with benign gastric diseases(P<0.05);(2)Pearson correlation analysis showed that there was a negative correlation between serum lncRNA-MIR100HG and miR-100(r=-0.483,P<0.05);(3)Single factor chi square analysis showed that the relative expression level of serum lncRNA-MIR100HG in gastric cancer patients was correlated with tumor size,differentiation,depth of tumor invasion,clinical stage,lymph node metastasis and distant metastasis,and the relative expression level of serum miR-100 was correlated with tumor size,differentiation,clinical stage and lymph node metastasis(P<0.05);(4)PFS of gastric cancer patients with low level expression of serum lncRNA-MIR100HG and miR-100 was longer than that of patients with high level expression(χ^(2)=37.371,P<0.05),PFS of gastric cancer patients with high miR-100 expression was longer than that of patients with low miR-100 expression(χ^(2)=28.631,P<0.05).Conclusion:The expression lev

关 键 词：胃癌 外泌体 lncRNA-MIR100HG miR-100 病理特征 无进展生存 预后 

分 类 号：R735.2[医药卫生—肿瘤]