靶向细胞器的次氯酸/次氯酸盐的小分子荧光探针研究进展  

Recent Advances of Organelle-Targeted Fluorescent Probes for Hypochlorous Acid/Hypochlorite

作　　者：李林柯 徐俊红[2] 麻秋娟[1] 朱楠楠 刘淑珍 LI Lin-ke;XU Jun-hong;MA Qiu-juan;ZHU Nan-nan;LIU Shu-zhen(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Department of Electrical Engineering,North China University of Water Resources and Electric Power,Zhengzhou 450011,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]华北水利水电大学电气工程学院,河南郑州450011

出　　处：《化学试剂》2023年第7期25-33,共9页Chemical Reagents

基　　金：国家自然科学基金项目(21807027)。

摘　　要：次氯酸(HClO)/次氯酸盐(ClO-)是生物体内重要的活性氧(ROS)之一,其浓度水平与生物体生理和病理进程密切相关。荧光成像分析法由于具有灵敏度高、时空分辨率高和原位实时等优点,已被广泛用于细胞内HClO/ClO-的检测。为了在亚细胞水平探究HClO/ClO-在相关生理病理过程中的作用机制,科研工作者开发了许多细胞器靶向HClO/ClO-的荧光探针。对近五年细胞器靶向HClO/ClO-的用于生物成像的小分子荧光探针进行综述,阐述这些荧光探针的设计原理、检测机制和生物学应用,为细胞器靶向HClO/ClO-荧光探针的开发和应用提供参考。Hypochloric acid(HClO)/hypochlorite(ClO-)is one of the important reactive oxygen species(ROS)in organisms,and its concentration level is closely related to the physiological and pathological processes of organisms.Fluorescence imaging analysis has been widely used for the detection of intracellular HClO/ClO-due to its advantages of high sensitivity,high spatiotemporal resolution,and real-time in situ.In order to explore the mechanism of HClO/ClO-in related physiological and pathological processes at the subcellular level,researchers have developed many organelle-targeted HClO/ClO-fluorescent probes.This article mainly reviewed the latest progress of organelle-targeted fluorescent probes for HClO/ClO-in the past five years.The design principle,detection mechanism,and biological applications of these fluorescent probes were described in detail,providing references for the development and application of organelle-targeted fluorescent probes for the detection of HClO/ClO-.

关 键 词：次氯酸/次氯酸盐 细胞器 荧光探针 研究进展 生物成像 

分 类 号：O657.3[理学—分析化学]