circ_0001721/miR-198对前列腺癌细胞生物学行为的影响  

The effect of circ_0001721/miR-198 on the biological behavior of prostate cancer cells

作　　者：王红梅 谢斌[2] 陈寒超[1] 关虹 李春芸[1] WANG Hongmei;XIE Bin;CHEN Hanchao;GUAN Hong;LI Chunyun(Department of Laboratory Medicine,Haikou Affiliated Hospital of Central South University Xiangya School of Medicine,Hainan Haikou 570203,China;Department of Critical Care Medicine,Haikou Affiliated Hospital of Central South University Xiangya School of Medicine,Hainan Haikou 570203,China)

机构地区：[1]中南大学湘雅医学院附属海口医院检验科,海南海口570203 [2]中南大学湘雅医学院附属海口医院重症医学科,海南海口570203

出　　处：《现代肿瘤医学》2023年第14期2575-2580,共6页Journal of Modern Oncology

基　　金：海南省卫生计生行业科研项目(编号:19A200164)。

摘　　要：目的:探讨circ_0001721/miR-198分子轴对人前列腺癌细胞DU145生物学行为的影响及相关作用机制。方法:应用qRT-PCR法检测circ_0001721、miR-198的表达量;DU145细胞转染si-circ_0001721、miR-198 mimics、anti-miR-198以及相应的对照(si-NC、miR-NC、anti-miR-NC);CCK-8法、平板克隆形成实验、流式细胞术、Transwell实验检测细胞增殖、克隆、凋亡、迁移及侵袭;双荧光素酶报告实验验证circ_0001721与miR-198的靶向关系;Western blot检测E-cadherin、N-cadherin蛋白表达量。结果:与癌旁组织比较,circ_0001721在前列腺癌组织中高表达(P<0.05),而miR-198在前列腺癌组织中低表达(P<0.05);与转染si-NC或转染miR-NC相比,si-circ_0001721或miR-198 mimics可以提高细胞增殖抑制率、凋亡率和E-cadherin蛋白水平(P<0.05),降低克隆形成数、迁移和侵袭数以及N-cadherin蛋白水平(P<0.05);circ_0001721可靶向结合miR-198;与共转染si-circ_0001721和anti-miR-NC相比,共转染si-circ_0001721和anti-miR-198降低了细胞增殖抑制率、凋亡率和E-cadherin蛋白水平(P<0.05),而增加了克隆形成数、迁移和侵袭数以及N-cadherin蛋白水平(P<0.05)。结论:敲低circ_0001721可通过上调miR-198抑制前列腺癌细胞的生长和转移。Objective:To determine the impact of circ_0001721/miR-198 molecular axis on the biological behavior of human prostate cancer cells DU145 and its related mechanism.Methods:The expression levels of circ_0001721 and miR-198 were detected by qRT-PCR.DU145 cells were transfected with si-circ_0001721,miR-198 mimics,anti-miR-198 and their negative controls(si-NC,miR-NC,anti-miR-NC),respectively.Cell proliferation,cloning,apoptosis,migration and invasion were assessed using CCK-8,colony formation assay,flow cytometry and Transwell assay,respectively.Targeting between circ_0001721 and miR-198 was validated using dual-luciferase reporter assay.The protein expression of E-cadherin and N-cadherin was detected by Western blot.Results:Compared with normal tissue,circ_0001721 expression in prostate cancer tissue was increased(P<0.05),while miR-198 expression in prostate cancer tissue was decreased(P<0.05).Compared with transfection of si-NC or miR-NC,the cell proliferation inhibition rate,apoptosis rate and the protein level of E-cadherin were increased(P<0.05),while the number of cell clone formation,migration,invasion and the protein level of N-cadherin were decreased(P<0.05)after transfection of si-circ_0001721 or miR-198 mimics.circ_0001721 could target miR-198.Compared with co-transfection with si-circ_0001721 and anti-miR-NC,the cell proliferation inhibition rate,apoptosis rate and the protein level of E-cadherin were reduced(P<0.05),while the number of cell clone formation,migration,invasion and the protein level of N-cadherin were increased(P<0.05)after co-transfection with si-circ_0001721 and anti-miR-198.Conclusion:Inhibition of circ_0001721 expression could reduce prostate cancer cell growth and metastasis by up-regulating miR-198.

关 键 词：前列腺癌 circ_0001721 miR-198 增殖 迁移 侵袭 凋亡 

分 类 号：R737.25[医药卫生—肿瘤]