赤红球菌提取物的制备及其化学成分分析  

Preparation of Rhodococcus ruber Extract and Analysis of Its Chemical Composition

作　　者：程贤 陈洲琴 张祝兰[1] 杨煌建[1] 严凌斌[1] 王德森[1] 连云阳[1] CHENG Xian;CHEN Zhou-qin;ZHANG Zhu-lan;YANG Huang-jian;YAN Ling-bin;WANG De-sen;LIAN Yun-yang(Fujian Institute of Microbiology/Fujian Key Laboratory of New Drugs(Microbiology)Screening,Fuzhou,Fujian 350007,China)

机构地区：[1]福建省微生物研究所/福建省新药(微生物)筛选重点实验室,福建福州350007

出　　处：《福建农业科技》2023年第4期42-47,共6页Fujian Agricultural Science and Technology

基　　金：福建省科技计划公益类项目(2020R10050014、2020R10050016)。

摘　　要：为建立赤红球菌提取物制备新工艺,以赤红球菌Rhodococcus ruber FIM 12为试验材料,通过单因素试验优化赤红球菌提取物制备条件,并进一步分析其类脂化合物、总糖、氨基酸等主要成分。结果表明:赤红球菌提取物制备收率为100 g湿细胞可制备3.97 g赤红球菌提取物,其类脂化合物含量为17.48%,多糖含量44.1%,含有1种氨糖(细胞壁特征氨基酸)和18种氨基酸(其中包含8种人体必需氨基酸),种类较为丰富。研究结果可为深入开发利用赤红球菌提取物可供参考。In order to establish a new process for the preparation of Rhodococcus ruber extract,by taking Rhodococcus ruber FIM-12 as the experimental material,the preparation conditions of Rhodococcus ruber extract were optimized by using the single factor test,and the main components such as lipoic acid,total polysaccharide and amino acids were further analyzed.The results showed that:the preparation yield of Rhodococcus ruber extract was as follows:3.97 g Rhodococcus ruber extract could be prepared from 100 g wet cells,which contained 17.48%lipoic acids and 44.1%polysaccharides.It contained 1 kind of glucosamine(cell wall characteristic amino acid)and 18 kinds of amino acids(including 8 kinds of essential amino acids),with relatively rich species.The research results could provide reference for the further development and utilization of Rhodococcus ruber extract.

关 键 词：赤红球菌 制备 多糖 氨基酸 

分 类 号：Q936[生物学—微生物学]