紫斑牡丹花粉化学成分及其体外抗氧化活性  

Chemical constituents from the pollen of Paeonia rockii and their in vitro antioxidant activities

作　　者：王新娣[1] 石晓峰[1] 刘东彦[1] 许晓辉 马潇 沈薇[1] 马趣环[1] 范彬[1] WANG Xin-di;SHI Xiao-feng;LIU Dong-yan;XU Xiao-hui;MA Xiao;SHEN Wei;MA Quan-huan;FAN Bin(Gansu Academy of Medical Science,Lanzhou 730050,China;Lanzhou Institutes for Food and Drug Control,Lanzhou 730030,China;Gansu Provincial Institute of Drug Control,Lanzhou 730070,China)

机构地区：[1]甘肃省医学科学研究院,甘肃兰州730050 [2]兰州市食品药品检验检测研究院,甘肃兰州730030 [3]甘肃省药品检验研究院,甘肃兰州730070

出　　处：《中成药》2023年第6期1858-1863,共6页Chinese Traditional Patent Medicine

基　　金：国家药品监督管理局中药材及饮片质量控制重点实验室项目(2020GSMPA-KL15);兰州市科技发展指导性计划项目(2019-ZD-132);甘肃省医学科学研究院中青年科技人才创新工程项目(ykz-2018-2)。

摘　　要：目的研究紫斑牡丹Paeonia rocki i(S.G.Haw&Lauener)T.Hong&J.J.Li花粉化学成分及其体外抗氧化活性。方法紫斑牡丹花粉70%乙醇提取物的正丁醇部位采用硅胶柱、反相硅胶C 18柱、ODS、聚酰胺、Sephadex LH-20进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用DPPH法评价其抗氧化活性。结果从中分离得到15个化合物,分别鉴定为柠檬黄素(1)、木犀草素-7-O-β-D-吡喃葡萄糖苷(2)、野漆树苷(3)、芹菜素-7-O-β-D-吡喃葡萄糖苷(4)、柠檬黄素-3-β-D-葡萄糖苷(5)、limocitrin-3-O-y1-β-D-sophoroside(6)、柯伊利素-7-O-β-D-吡喃葡萄糖苷(7)、白芍苷R1(8)、苯甲酰芍药苷(9)、白藜芦醇(10)、苯甲酸(11)、3-甲氧基-4-羟基苯甲酸(12)、没食子酸甲酯(13)、胡萝卜苷(14)、1,2,3,4,6-五没食子酰葡萄糖(15)。化合物1~2、10、15的IC 50值均低于0.02 mg/L,化合物15的IC 50值小于抗环血酸,化合物2的IC 50值与抗环血酸相当。结论化合物2~4、7~10、12、15均为首次从该植物中分离得到。化合物1~7、10、12~13、15均表现出一定的体外抗氧化性。AIM To study the chemical constituents from the pollen of Paeonia rocki i(S.G.Haw&Lauener)T.Hong&J.J.Li and their in vitro antioxidant activities.METHODS The n-butanol fraction of 70%ethanol extract from the pollen of P.rockii was isolated and purified by silica gel column,reverse-phase silica gel C 18 column,ODS,polyamide and Sephadex LH-20,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The in vitro antioxidant activities were evaluated by DPPH method.RESULTS Fifteen compounds were isolated and identified as limocitrin(1),luteolin-7-O-β-D-glucopyranoside(2),rhoifolin(3),apigenin-7-O-β-D-neospheroside(4),limocitrin-3-β-D-glucoside(5),limocitrin-3-O-y1-β-D-sophoroside(6),chrysoeriol-7-O-β-D-glucopyranoside(7),albiflorin R1(8),benzoylpaeoniflorin(9),resveratrol(10),benzoic acid(11),3-methoxy-4-hydroxybenzoic acid(12),methyl gallate(13),β-daucosterin(14),1,2,3,4,6-pentagalloylglucose(15).The IC 50 values of compounds 1-2,10,15 were lower than 0.02 mg/L.The IC 50 value of compound 15 was less than that vitamin C.The IC 50 value of compound 2 was the same with vitamin C.CONCLUSION Compounds 2-4,7-10,12,15 are isolated from this plant for the first time.Compounds 1-7,10,12-13,15 show certain in vitro antioxidant activities.

关 键 词：紫斑牡丹 花粉 化学成分 抗氧化活性 分离鉴定 

分 类 号：R284.1[医药卫生—中药学]