rhGLP-1(7-36)对肝细胞Akt/GSK3信号通路的影响  

Effects of rhGLP-1(7-36)on Akt/GSK3 signaling pathway in hepatocytes

作　　者：边志颖 张永青[2] 王宏宇[1] 高飞[1] 姚瑶 王意 朱泊羽 徐春[1] Bian Zhiying;Zhang Yongqing;Wang Hongyu;Gao Fei;Yao Yao;Wang Yi;Zhu Boyu;Xu Chun(Department of Endocrinology,the Third Medical Center of PLA General Hospital,Beijing 100039,China;Department of Gerontology,the Third Medical Center of PLA General Hospital,Beijing 100039,China)

机构地区：[1]解放军总医院第三医学中心内分泌科,北京100039 [2]解放军总医院第三医学中心老年科,北京100039

出　　处：《中华内分泌外科杂志》2023年第3期291-294,共4页Chinese Journal of Endocrine Surgery

基　　金：武警总医院科研项目 (WZ20130104)。

摘　　要：目的观察rhGLP-1(7-36)对肝细胞Akt/GSK3信号通路的影响。方法培养人HL7702细胞系至对数生长期,将其分为实验组和空白对照组,分别用含rhGLP-1(7-36)100nM的培养基、不含rhGLP-1(7-36)的培养基培养90min。用蛋白质免疫印迹法(Western blot)检测两组Akt、糖原合成酶激酶3(glycogen synthase kinase,GSK3)、糖原合酶(glycogen synthase,GS)水平。结果与空白对照组(1.00±0.00)比,实验组p-Akt(Thr308)蛋白相对表达水平(1.81±0.28)明显增加(P=0.01),Akt及p-Akt(Ser473)蛋白表达水平无明显变化;实验组p-GSK3α(Ser21)(1.27±0.09)、p-GSK3β(Ser9)(1.24±0.09)蛋白表达水平明显升高(P值分别为0.003、0.002),GSK3α及GSK3β蛋白表达水平无明显变化;实验组p-GS(Ser641)蛋白表达水平(0.70±0.16)降低(P=0.03),GS蛋白表达水平无明显变化。结论GLP-1可抑制GSK3/GS信号通路,激活GS活性,促进糖原合成。Objective To observe the effect of rhGLP-1(7-36)on Akt/GSK3 signaling pathway in hepatocytes.Methods Human HL7702 cell line was cultured to the logarithmic growth stage and divided into experimental group and blank control group.The cultures were incubated with 100nM medium containing rhglp-1(7-36)and without rhglp-1(7-36)for 90min.The levels of Akt,Glycogen synthase Kinase 3(GSK3)and Glycogen synthase(GS)in the two groups were detected by Western Blot.Results Compared with blank control group,the protein expression of p-Akt(Thr308)in experimental group(1.81±0.28)was significantly increased(P=0.01),but the protein expression of Akt and p-Akt(Ser473)was not significantly changed.The protein expression levels of p-GSK3α(Ser21)(1.27±0.09)and p-GSK3β(Ser9)(1.24±0.09)in the experimental group were significantly increased(P=0.003,0.002),while the protein expression levels of GSK3αand GSK3βwere not significantly changed.The protein expression level of p-GS(Ser641)(0.70±0.16)was decreased in the experimental group(P=0.03),but the protein expression level of GS did not change significantly.Conclusion Glp-1 can inhibit GSK3/GS signaling pathway,activate GS activity and promote glycogen synthesis.

关 键 词：肠促胰岛素 AKT 胰岛素信号通路 人肝HL7702细胞系 

分 类 号：R575[医药卫生—消化系统]