培养液中添加硫酸链霉素对蛋白核小球藻生长、细胞组成及α-亚麻酸含量的影响  

Effects of supplementation of streptomycin sulfate in culture medium on growth,cellular biochemical composition andα-Linolenic acid content of Chlorella pyrenoidosa

作　　者：穆亮亮 黄旭雄[1,2,3] MU Liangliang;HUANG Xuxiong(Centre for Research on Environmental Ecology and Fish Nutrition,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China;Shanghai Engineering Research Center of Aquaculture,Shanghai 201306,China;Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学农业农村部鱼类营养与环境生态研究中心,上海201306 [2]上海海洋大学水产种质资源发掘与利用教育部重点实验室,上海201306 [3]上海海洋大学水产科学国家级实验教学示范中心,上海201306

出　　处：《上海海洋大学学报》2023年第3期522-531,共10页Journal of Shanghai Ocean University

基　　金：沪科技兴农项目(2021-02-08-00-12-F00761)。

摘　　要：在培养液中分别添加0(对照组)、20、60、180和540μg/mL的硫酸链霉素(STRS),研究STRS作用下蛋白核小球藻SHOU-1002(Chlorella pyrenoidosa SHOU-1002)生长、细胞组成及α-亚麻酸含量。结果表明:前6天,对照组藻细胞密度显著高于各STRS处理组,第10天对照组生物量及单个细胞干质量均显著大于各STRS处理组。STRS处理组的生物量随培养液中STRS质量浓度的升高不断降低。对照组、20、60和180μg/mL STRS处理组藻细胞叶绿素a、叶绿素b和类胡萝卜素含量均随培养时间的延长先降低后升高;540μg/mL STRS处理组藻细胞叶绿素a和叶绿素b含量随培养时间的延长持续下降,而类胡萝卜素前4天快速下降随后趋于稳定。α-亚麻酸是蛋白核小球藻SHOU-1002细胞脂肪酸组成中的优势脂肪酸,且4天后各STRS处理组藻细胞中α-亚麻酸含量显著高于对照组。第10天,180μg/mL STRS处理组藻细胞α-亚麻酸的绝对含量最高(52.09 mg/g),较对照组提升了108.19%;同时180μg/mL STRS处理组的α-亚麻酸产量(35.34 mg/L)也最高。藻细胞色素(%DW)和脂肪酸(%)的相关性分析显示α-亚麻酸含量和叶绿素a含量呈极显著负相关。结果表明,培养液中STRS降低蛋白核小球藻SHOU-1002叶绿素a含量和生物量的同时促进了藻细胞中α-亚麻酸的蓄积。The growth,cellular biochemical composition,andα-Linolenic acid content of Chlorella pyrenoidosa SHOU-1002 treated with streptomycin sulfate(STRS)at concentrations of 0(CK),20,60,180 and 540μg/mL were examined.The results showed that the microalgal cell density in the CK group was significantly higher than those in STRS treated groups in the first 6 days.The biomass and single cell weight of the CK group on the 10th day were significantly higher than those of STRS treated groups.The biomass of STRS treated group decreased continuously with the increasing of STRS concentration in medium.The contents of Chlorophyll a,Chlorophyll b,and carotenoids in microalgae of CK,20,60,and 180μg/mL STRS groups,firstly decreased then increased with prolongation of the culture time.The contents of Chlorophyll a and Chlorophyll b continuously declined while carotenoids sharply decreased in the first 4 days then stabilized in the treatments of 540μg/mL STRS group.During the whole culture period,α-Linolenic acid was the dominant in the fatty acid profiles of C.pyrenoidosa SHOU-1002.Theα-Linolenic acid contents(%)in STRS treated groups were significantly higher than that in the CK group since the 4th day.On the 10th day,the microalgae in 180μg/mL STRS group had the highest content ofα-Linolenic acid(52.09 mg/g),which increased by 108.19%as compared to that of the CK group.Moreover,the microalgae in 180μg STRS/mL group also had the highestα-Linolenic acid yield(35.34 mg/L).The correlation analysis illustrated thatα-Linolenic acid content and chlorophyll a content of the microalgae was negatively,highly,and significantly correlated.It is therefore suggested that STRS in culture medium decreased the chlorophyll a content and biomass,but promoted the accumulation ofα-Linolenic acid in C.pyrenoidosa SHOU-1002.

关 键 词：蛋白核小球藻 硫酸链霉素 生长 色素 细胞组成 Α-亚麻酸 

分 类 号：S968.4[农业科学—水产养殖]