含ACC脱氨酶的植物促生菌对荷花耐镉性及镉富集的影响  

Effects of plant growth-promoting bacteria containing ACC deaminase on cadmium tolerance and cadmium enrichment in lotus

作　　者：江婷 李泽鑫 胡途 徐迎春[1] 金奇江 王彦杰[1] JIANG Ting;LI Zexin;HU Tu;XU Yingchun;JIN Qijiang;WANG Yanjie(College of Horticulture,Nanjing Agricultural University/Key Laboratory of Landscape Agriculture,Ministry of Agriculture and Rural Affairs/Key Laboratory of Flower Biology in East China,State Forestry and Grassland Administration/Key Laboratory of Flower Biology and Germplasm Creation,Ministry of Agriculture and Rural Affairs,Nanjing 210095,China)

机构地区：[1]南京农业大学园艺学院/农业农村部景观农业重点实验室/国家林业与草原局华东地区花卉生物学重点实验室/农业农村部花卉生物学与种质创制重点实验室,南京210095

出　　处：《农业环境科学学报》2023年第6期1379-1387,共9页Journal of Agro-Environment Science

基　　金：江苏省农业科技自主创新资金项目(CX(21)3018);国家自然科学基金项目(32071829,31772346,31971710)。

摘　　要：为研究含ACC脱氨酶的植物促生菌(PGPB)对改善荷花耐Cd性及植物修复效率的作用,以观赏荷花品种“秣陵秋色”(Nelumbo nucifera‘Molingqiuse’)为材料,通过盆栽试验探讨了Cd胁迫条件下,接种含ACC脱氨酶的PGPB(Pantoea vagans So23和Pseudomonas veronii E02)对荷花的生长、生理代谢及Cd吸收积累能力的影响。结果显示:接种So23和E02能够有效缓解Cd胁迫对荷花生长的抑制作用,使得株高、叶面积及立叶数与未接菌Cd处理相比均有不同程度的增加,叶片中丙二醛含量分别降低38.39%和31.21%,抗坏血酸含量分别升高54.85%和36.64%。接种So23和E02显著改变了Cd在荷花不同器官间的分配模式,能够通过限制Cd从地下部向地上部叶片的转移来抵抗Cd胁迫;此外,接种So23能明显增强荷花对底泥中Cd的富集,使底泥中Cd含量下降56.47%,改善了底泥环境。研究表明,接种So23和E02在一定程度上能够通过提高抗氧化剂含量增强荷花对Cd胁迫的耐受性,同时改变荷花对Cd的转运及富集能力。To investigate the effects of plant growth-promoting bacteria(PGPB)containing ACC deaminase on improving Cd tolerance and remediation efficiency in lotus,a pot experiment was conducted to analyze the influence of Pantoea vagans So23 and Pseudomonas veronii E02 inoculation on the growth,physiological metabolism,and Cd absorption and accumulation capacity of the ornamental lotus variety‘Molingqiuse’under Cd stress.The results showed that,compared with the Cd treatment alone,inoculation of So23 and E02 effectively alleviated the inhibition of lotus growth under Cd stress,and led to an increase in plant height,leaf area,and total number of vertical leaves.MDA content in leaves was decreased by 38.39%and 31.21%,respectively,under Cd stress combined with So23 and E02 inoculation,but the ascorbic acid was increased by 54.85%and 36.64%,respectively,after the supplementation.The two strains significantly changed the distribution pattern of Cd in different lotus organs,and could resist Cd stress by limiting Cd-transport from underground organs to leaves.Additionally,the strain So23 markedly enhanced the Cd enrichment in lotus,and the Cd content in the sediment environment was decreased by 56.47%,and improved the sediment environment.The research shows that supplementation with So23 and E02 can,to some extent,improve Cd-stress tolerance in lotus by increasing antioxidant content and changing its Cd translocation and enrichment capacity.

关 键 词：植物促生菌(PGPB) ACC脱氨酶 镉胁迫 莲 促生效应 生物富集 

分 类 号：X171.5[环境科学与工程—环境科学]