Roots and rhizomes of wild Asparagus:Nutritional composition,bioactivity and nanoencapsulation of the most potent extract  

作　　者：Khaoula Adouni Ana Júlio Celestino Santos-Buelga Ana M.Gonzalez-Paramas Patrícia Filipe Patricia Rijo Sofia A.Costa Lima Salette Reis Angela Fernandes Isabel C.F.R.Ferreira Virginia Fernandez-Ruiz Patricia Morales Guido Flamini Lotfi Achour Pedro Fonte 

机构地区：[1]Laboratoire de Recherche“Bioressources:Biologie Integrative&Valorisation”,Institut Superieur de Biotechnologie de Monastir,Avenue Tahar Hadded,BP 74,Universite de Monastir,5000,Monastir,Tunisia [2]CBIOS-Research Center for Biosciences&Health Technologies,Universidade Lusofona de Humanidades e Tecnologias,Campo Grande 376,1749-024,Lisbon,Portugal [3]Department of Biomedical Sciences,University of Alcal´a,Ctra.Madrid-Barcelona Km.33.600,28871,Alcala de Henares,Madrid,Spain [4]Grupo de Investigacion en Polifenoles(GIP-USAL),Facultad de Farmacia,Universidad de Salamanca,Campus Miguel de Unamuno,E-37007,Salamanca,Spain [5]Research Institute for Medicines(iMed.ULisboa),Faculty of Pharmacy,Universidade de Lisboa,Av.Professor Gama Pinto,1649-003,Lisboa,Portugal [6]LAQV,REQUIMTE,Department of Chemical Sciences–Applied Chemistry Lab,Faculty of Pharmacy,10 University of Porto,Rua Jorge de Viterbo Ferreira 228,4050-313,Porto,Portugal [7]Centro de Investigacao de Montanha(CIMO),Instituto Politecnico de Braganca,Campus de Santa Apolonia,5300-253,Braganca,Portugal [8]Department of Nutrition and Food Science,Faculty of Pharmacy,Complutense University of Madrid(UCM),Plaza Ramon y Cajal s/n,28040,Madrid,Spain [9]Dipartimento di Farmacia,University of Pisa,via Bonanno 6,56126,Pisa,Italy [10]Centro Interdipartimentale di Ricerca“Nutraceutica e Alimentazione per la Salute”University of Pisa,Via del Borghetto 80,56124,Pisa,Italy [11]Center for Marine Sciences(CCMar),University of Algarve,Gambelas Campus,8005-139,Faro,Portugal [12]Department of Chemistry and Pharmacy,Faculty of Sciences and Technology,University of Algarve,Gambelas Campus,8005-139,Faro,Portugal [13]iBB-Institute for Bioengineering and Biosciences,Department of Bioengineering,Instituto Superior Tecnico,Universidade de Lisboa,1049-001,Lisboa,Portugal [14]Associate Laboratory i4HB—Institute for Health and Bioeconomy at Instituto Superior Tecnico,Universidade de Lisboa,Av.Rovisco Pais,1049-001,Lisboa,Portugal

出　　处：《Food Bioscience》2022年第1期349-360,共12页食品生物科学（英文）

基　　金：ALIMNOVA Research Group(UCM GR105/18);Spanish Government through the project PID2019-109365RA-I00;Foundation for Science and Technology(FCT,Portugal)for financial support through national funds FCT/MCTES to CIMO(UIDB/00690/2020);LAQV(UIDB/50006/2020);CCMar(UIDB/04326/2020);CBIOS(UIDB/04567/2020);iBB-IST(UIDB/04565/2020);National funding by FCT,P.I.,through the institutional scientific employment program-contract;FEDER-Interreg Espana-Portugal programme for financial support through the project 0377_Iberphenol_6_E;financially supported by the Spanish Government through the project AGL2015-64522-C2-2-R.

摘　　要：The nutritional composition and bioactive properties of roots and rhizomes of Asparagus stipularis were evaluated.Antioxidant activity of extracts obtained by infusion was evaluated using free radicals scavenging and reducing power methods.Porcine liver primary cell was used to check the hepatotoxicity of infusions.Results revealed that Asparagus samples are likely a source of nutrients,such as dietary fibre and essential fatty acids.HPLC-DAD-ESI/MS characterization of infusions allowed the identification and quantitation of 7 phenolic compounds,all hydroxycinnamoyl derivatives,with caffeic acid as the most abundant.Roots infusion contained the highest amounts of these compounds.It also exhibited the highest antioxidant activity in all assays,with EC_(50) values of 0.44±0.01,0.98±0.03 and 0.64±0.01 mg/mL for DPPH,ABTS and FRAP assays,respectively,with no toxicity towards PLP2 primary cell cultures(GI_(50)>400μg/mL).PLGA nanoparticles loaded with root extract were prepared using solvent-evaporation double emulsion method.Nanoparticles size was about 260 nm and a polydispersity index around 0.1,with a zeta potential of about-36 mV,as well as a good encapsulation efficiency of approximately 83%.Their morphology was analysed by SEM and spherical polymeric nanoparticles with a smooth surface were observed.FTIR and DSC were also performed,which allowed corroborating the efficacy of the encapsulation and to confirm the production of a stable and robust system to load Asparagus extracts.The developed nanoparticles are expected to be used as delivery systems for bioactive compounds of A.stipularis and they could be used as an innovative dietary supplement.

关 键 词：Asparagus stipularis Nutritional composition Antioxidant activity Nanoparticles PLGA 

分 类 号：TS201.2[轻工技术与工程—食品科学]