脑缺血再灌注损伤大鼠肠道菌群的变化及功能分析  

Changes and functional analysis of gut microbiota in rats with cerebral ischemia-reperfusion injury

作　　者：梁国晶 冀琨 任海燕 张恺纯 张钰鸽 文娟 LIANG Guojing;JI Kun;REN Haiyan;ZHANG Kaichun;ZHANG Yuge;WEN Juan(Basic Medical College,Xinjiang Medical University,Urumqi 830011,China;不详)

机构地区：[1]新疆医科大学基础医学院病理生理学教研室,乌鲁木齐830011 [2]新疆医科大学第六附属医院神经外科 [3]新疆医科大学实验室与设备管理处中心实验室 [4]乌鲁木齐市友谊医院心内科 [5]长葛市人民医院病理科

出　　处：《山东医药》2023年第19期23-26,共4页Shandong Medical Journal

基　　金：新疆维吾尔自治区自然科学基金项目(2019D01C191)。

摘　　要：目的探讨脑缺血再灌注损伤(CIRI)大鼠的肠道菌群变化及其对CIRI的影响机制。方法将48只SD大鼠按随机数字表法分为Sham组、Model组各24只。Model组用线栓法阻断大鼠大脑中动脉血流2 h后恢复再灌注7 d建立大脑中动脉阻断模型,Sham组大鼠仅分离颈总动脉。再灌注7 d后,两组采集粪便标本,用16S rRNA测序技术进行肠道菌群丰度、多样性和差异性分析,用PICRUSt2分析差异菌群的功能。结果两组肠道菌群Alpha多样性比较差异无统计学意义(P>0.05),两组肠道菌群结构比较差异有统计学意义(P<0.05)。与Sham组比较,Model组乳酸杆菌属、颤螺菌属丰度低(P均<0.05),消化链球菌科、肠杆菌科等丰度高(P均<0.05)。差异菌群的功能在代谢通路富集,神经变性疾病、萜类、聚酮在Model组显著聚集(P均<0.05)。结论CIRI大鼠肠道菌群紊乱,有益菌中的乳酸杆菌属、颤螺菌属丰度减少,有害菌中的肠杆菌科丰度增高;肠道菌群可能通过代谢途径中的神经变性疾病、萜类、聚酮促进CIRI。Objective To investigate the changes of gut microbiota in rats with cerebral ischemia-reperfusion injury(CIRI)and its mechanism.Methods Forty-eight SD rats were randomly divided into the Sham group and Model group,with 24 rats in each group.We established the middle cerebral artery occlusion(MCAO)models in the Model group by blocking the middle cerebral artery blood flow in rats for 2 h and then resumed the reperfusion for 7 days.In the Sham group,we only separated the common carotid arteries of rats.After 7 days of reperfusion,fecal samples were collected from the two groups,and 16S rRNA gene sequencing technology was used to analyze the abundance,diversity and differ-ence of gut microbiota,and PICRUSt2 was used to predict the function of different flora.Results There was no signifi-cant difference in the Alpha diversity of gut microbiota between the two groups(P>0.05),but there was significant differ-ence in gut microbiota structure between the two groups(P<0.05).Compared with the Sham group,Model group had low-er abundances of Lactobacillus and Oscillospira(both P<0.05),and higher abundances of Peptostreptococcaceae and En-terobacteriaceae(both P<0.05).The function of differential microbiota was enriched in metabolic pathways,and Neurode-generative diseases,terpenoids,and poly ketones were aggregated in the Model group(all P<0.05).Conclusions The gut microbiota of CIRI rats is disordered,the abundances of Lactobacillus and Oscillospira in beneficial bacteria decrease,and the abundance of Enterobacteriaceae in harmful bacteria increases.Gut microbiota may promote CIRI through neurode-generative diseases,terpenoids and poly ketones in the metabolic pathway.

关 键 词：脑缺血再灌注损伤 肠道菌群 16S rRNA基因测序技术 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]