13味中草药提取液抑菌及抑饲料霉变的研究  

Study on Bacteriostasis and Feed Mildew Inhibition of 13 Kinds of Chinese Herbal Medicine Extracts

作　　者：邢玉薪 褚福壮 李姣玉 姚悦璠 李艺 杨艳[1] 白文正 XING Yuxin;CHU Fuzhuang;LI Jiaoyu;YAO Yuefan;LI Yi;YANG Yan;BAI Wenzheng(College of Chemical Engineering and Technology,Taiyuan University of Science and Technology,Taiyuan 030006,China;Shanxi Anyou Biological Technology Co.,Ltd.,Jinzhong 030600,China)

机构地区：[1]太原科技大学化学工程与技术学院,山西太原030006 [2]山西安佑生物科技有限公司,山西晋中030006

出　　处：《海南师范大学学报（自然科学版）》2023年第2期192-197,共6页Journal of Hainan Normal University（Natural Science）

基　　金：山西省高等学校大学生创新创业训练计划项目(20210472);山西省省级教学改革项目(J2021422)。

摘　　要：研究了玫瑰、白芍、蒲公英等13味中草药提取液的抑菌及抑饲料霉变的效果。首先利用索氏提取法分别提取13味中草药有效成分,采用纸片扩散法测定提取物对金黄色葡萄球菌和大肠杆菌的抑制作用,显微镜下观察提取物对青霉的菌丝及孢子发育的影响,进一步筛选出抑菌效果较好的几味中草药进行混合提取同时进行抑饲料霉变的分析。结果显示当菌液浓度为106 cfu/mL时,13味中草药提取液均对受试菌株有不同程度的抑菌作用,其中地榆和玫瑰提取液的抑菌效果较为明显,相对抑菌直径均不低于1.25 cm,并且地榆提取物能显著破坏青霉孢子。同时地榆、党参等几味中草药混合物在与饲料混合储存15 d内可有效抑制饲料霉变。本研究结果为相关中草药抑菌机制的研究及其在天然防腐剂领域的应用等方面提供了研究基础。The antibacterial and mildew inhibiting effects of 13 kinds of Chinese herbal medicines,including Rosa ru⁃gosa Thunb.,Paeonia lactiflora Pall and Taraxacum mongolicum Hand.-Mazz were studied in this paper.The effective components of 13 herbal medicines were extracted by the Soxhlet extraction method firstly.After extraction,the inhibitory effects on E.coli and S.aureus were determined using the Disk Diffusion Test,and the effects on the hypha and spore of penicillium were observed under the microscope.Then several Chinese herbal medicines with better antibacterial effects were selected for mixed extraction and analysis of inhibiting feed mildew.The result showed that at the bacterial concentration of 106 cfu/mL,13 Chinese herbal extracts had varying levels of antibacterial effects on the tested strains.Extracts from Sanguisorba Officinalis L and Rose had more pronounced antibacterial effects,with relative inhibitory diameters of no less than 1.25 cm,and the extract of Sanguisorba Officinalis L was found to disrupt the spore morphology of penicillium significantly.At the same time,the mixed extract of Chinese herbal medicines such as Sanguisorba Offici⁃nalis L and Radix Codonopsis was found to be effective in suppressing mildew in feed within 15 days of storage.These results provide a research foundation for the antibacterial mechanism of Chinese herbal medicine and its application in the field of natural preservatives.

关 键 词：中草药 抑菌 饲料霉变 

分 类 号：R284.2[医药卫生—中药学]