肌球蛋白重链在不同年龄间歇性外斜视患者内直肌中的表达  

Expression of myosin heavy chain in medial rectus muscle of patients with intermittent exotropia at different ages

作　　者：李芳 龙登虹 邓斯元 吴挺飞 LI Fang;LONG Denghong;DENG Siyuan;WU Tingfei(Department of Ophthalmology,Hainan Eye Hospital,Haikou,Hanan,China,570311)

机构地区：[1]海南省眼科医院眼科,海南海口570311

出　　处：《分子诊断与治疗杂志》2023年第6期930-933,共4页Journal of Molecular Diagnostics and Therapy

基　　金：海南省自然科学基金项目(820QN415);海南省卫生健康行业科研项目(20A200162)。

摘　　要：目的观察肌球蛋白重链(MyHC)在不同年龄间歇性外斜视患者内直肌中的表达。方法选取2020年9月至2022年9月于海南省眼科医院行斜视矫正手术的102例间歇性外斜视患者为研究对象。根据患者年龄分为儿童组(0~6岁)(n=36)、少年组(7~17岁)(n=35)、青年组(18~40岁)(n=31)。于斜视矫正手术中取患者内直肌标本进行研究,采用免疫组化染色、Western blot法和BCA法观察肌球蛋白、肌动蛋白、肌球蛋白重链13(MYH 13)、肌球蛋白重链1(MYH 1)在不同年龄间歇性外斜视患者内直肌中的表达,采用Spearman相关分析三者表达水平与年龄的相关性。结果MYH 13、肌球蛋白、肌动蛋白表达水平:儿童组>少年组>青年组,差异均具有统计学意义(F=217.743、6.087、36.871,P<0.05);3组MYH 1水平比较,差异无统计学意义(P>0.05)。Spearman性相关分析结果显示,MYH 13、肌球蛋白、肌动蛋白表达水平与年龄呈负相关(r=-0.361、-0.479、-0.262,P均<0.05)。结论低龄间歇性外斜视患者内直肌中MYH 13、肌球蛋白、肌动蛋白表达水平相对较高,且与患者年龄呈负相关。Objective To observe the expression of myosin heavy chain(MyHC)in the medial rectus muscle of patients with intermittent exotropia at different ages.Methods 102 patients with intermittent exotropia who underwent strabismus correction surgery in Hainan Eye Hospital from September 2020 to Septem⁃ber 2022 were selected as the study subjects.The patients were divided into the children group(0⁃6 years old)(n=36),the juvenile group(7⁃17 years old)(n=35)and the youth group(18⁃40 years old)(n=31)according to their age.The medial rectus muscle samples were taken from patients during strabismus correction surgery,and immunohistochemical staining,Western blot and BCA methods were used to observe myosin,actin,myo⁃sin heavy chain 13(MYH 13),myosin heavy chain Chain 1(MYH 1)was expressed in the medial rectus mus⁃cle of patients with intermittent exotropia of different ages,and the correlation between the three expression lev⁃els and age was analyzed by Spearman correlation.Results MYH 13,myosin and actin expression levels:chil⁃dren group>juvenile group>youth group,the difference was statistically significant(F=217.743,6.087,36.871,P<0.05).There was no statistically significant difference in MYH1 levels among the three groups(P>0.05).The results of Spearman's correlation analysis showed that the expression levels of MYH 13,myosin,and actin were negatively correlated with age(r=-0.361,-0.479,-0.262,P<0.05).Conclusion The expres⁃sion levels of MYH 13,myosin and actin in the medial rectus of young patients with intermittent exotropia are relatively high,and they are negatively correlated with the age of the patients.

关 键 词：肌球蛋白重链 肌动蛋白 间歇性外斜视 眼外肌 年龄 

分 类 号：R777.41[医药卫生—眼科]