肝细胞癌中ILF3蛋白过表达及其临床病理学意义  

Clinicopathological significance of ILF3 protein overexpression in hepatocellular carcinoma

作　　者：尹香琳 张卉[1] 刘启贤 吴焕文[1] YIN Xiang-lin;ZHANG Hui;LIU Qi-xian;WU Huan-wen(Department of Pathology,Chinese Academy of Medical Sciences,Peking Union Medical College and Peking Union Medical College Hospital,Beijing 100730,China)

机构地区：[1]中国医学科学院/北京协和医学院/北京协和医院病理科,北京100730

出　　处：《临床与实验病理学杂志》2023年第6期645-650,共6页Chinese Journal of Clinical and Experimental Pathology

基　　金：中央高水平医院临床科研资助项目(2022-PUMCH-B-063)。

摘　　要：目的探讨ILF3蛋白表达与肝细胞癌(hepatocellular carcinoma,HCC)临床病理特征及预后的关系。方法利用UALCAN和GEPIA生物信息数据库分析ILF3 mRNA在HCC和正常肝组织中的差异表达,分析其与HCC患者生存期的关系;应用免疫荧光染色检测ILF3蛋白在Huh-7 HCC细胞中的表达定位。采用免疫组化SP法检测ILF3蛋白在80例HCC和80例癌旁非瘤组织中的差异表达;应用χ^(2)检验分析ILF3蛋白表达与HCC临床病理特征的关系。应用Kaplan-Meier生存分析ILF3蛋白表达与HCC患者生存期的关系,采用Cox回归模型分析ILF3蛋白表达在HCC预后评估中的价值。结果数据库分析结果显示,ILF3 mRNA在HCC组织中的表达明显高于正常肝组织,且ILF3 mRNA高表达与HCC患者较短的生存期相关。免疫荧光检测显示,ILF3蛋白主要定位于细胞核。免疫组化检测显示,ILF3蛋白在HCC组织中的细胞核表达率和高表达率分别为81.3%(65/80)和60.0%(48/80),明显高于癌旁非瘤组织(41.3%,25.0%,P<0.01)。ILF3蛋白高表达与HCC肿瘤最大径(χ^(2)=5.291,P=0.024)、病理分级(χ^(2)=6.888,P=0.011)及临床分期(χ^(2)=5.160,P=0.031)明显相关。ILF3蛋白高表达HCC患者的总生存期短于ILF3蛋白低表达患者,且ILF3蛋白表达是HCC患者预后不良的独立风险因素(HR:2.148,95%CI=1.031~4.475,P=0.041)。结论ILF3蛋白表达与HCC发生、发展及预后密切相关,可作为HCC预后评估的潜在标志物。Purpose To explore the relationship between the expression level of ILF3 and the clinical pathological characteristics and prognosis of the patients with hepatocellular carcinoma(HCC).Methods UALCAN and GEPIA database were applied to analyze the expression of ILF3 mRNA in HCC,and to analyze the relationship between ILF3 mRNA expression and survival of the patients with HCC.The cellular localization of ILF3 protein were detected by using immunofluorescence(IF)staining.Then,immunohistochemical(IHC)staining was performed to detect the expression of ILF3 protein in 80 HCC tissues and 80 para-neoplastic non-tumor tissues,and the relationship between ILF3 expression and pathological parameters were analyzed.Additionally,the correlation between ILF3 and survival of HCC patients were determined by Kaplan-Meier analysis.Moreover,the prognostic value of ILF3 in HCC was evaluated by Cox-regression model.Results Bioinformatics results showed that ILF3 mRNA expression was significantly up-regulated in HCC tissues,and high expression of ILF3 mRNA was related to short survival of HCC patients.IF staining showed that ILF3 protein was mainly expressed in nucleolus of HCC cells.The IHC staining results showed that the positive and strong positive expression rate of ILF3 protein in HCC were 81.3%(65/80)and 60.0%(48/80),respectively,which were significantly higher than those in para-neoplastic non-tumor tissues(41.3%,25.0%,P<0.01).Chi-square test showed that the expression of ILF3 was related to tumor size(χ^(2)=5.291,P=0.024),histological grade(χ^(2)=6.888,P=0.011)and clinical stage(χ^(2)=5.160,P=0.031).Kaplan-Meier survival analysis showed that high expression of ILF3 related to shorter survival time.Cox regression analysis showed that ILF3 protein expression(HR=2.148,95%CI=1.031-4.475,P=0.041)were independent risk factors for HCC.Conclusion Elevated expression of ILF3 is closely related to the progression and prognosis of HCC.ILF3 may potentially be used as an important prognostic indicator for HCC.

关 键 词：肝肿瘤 ILF3 免疫组织化学 生存分析 预后 

分 类 号：R735.7[医药卫生—肿瘤]