幽门螺旋杆菌感染与MALT1基因易位对胃MALT淋巴瘤免疫微环境的影响及意义  

Effects of HP infection and MALT1 gene translocation on immune microenvironment of gastric MALT lymphoma and its clinical significance

作　　者：邵龙飞 许周毅 梁佳毅 张亮 王哲 SHAO Long-fei;XU Zhou-yi;LIANG Jia-yi;ZHANG Liang;WANG Zhe(Department of Pathology,the First Affiliated Hospital of University of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230036,China;Department of Pathology,Xijing Hospital,Air Force Medical University,Xi’an 710032,China)

机构地区：[1]中国科学技术大学生命科学与医学部附属第一医院病理科,合肥230036 [2]空军军医大学西京医院病理科,西安710032

出　　处：《临床与实验病理学杂志》2023年第6期650-654,共5页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探究幽门螺旋杆菌(helicobacter pylori,HP)感染和黏膜相关淋巴组织淋巴瘤易位基因1(mucosa-associated lymphoid tissue lymphoma translocation gene 1,MALT1)易位对胃黏膜相关淋巴组织(mucosa-associated lymphoid tissue,MALT)淋巴瘤免疫微环境及患者预后的影响。方法通过免疫组化染色检测40例胃MALT淋巴瘤患者石蜡活检样本中CD3^(+)T细胞、CD4^(+)T细胞、CD8^(+)T细胞、Foxp3^(+)调节性T细胞(Treg)、CD68^(+)巨噬细胞、CD163^(+)M2型巨噬细胞、CD83^(+)树突状细胞(dendritic cell,DC)、CD56^(+)自然杀伤细胞(natural killer cell,NK)和CD33^(+)髓源抑制细胞(myeloid-derived suppressor cells,MDSC)的浸润情况。利用HE染色对患者HP感染情况进行判定,同时采用FISH检测40例胃MALT淋巴瘤中MALT1基因易位情况,根据HP感染和MALT1基因易位情况进行分组,比较免疫微环境及患者预后的差别。结果40例胃MALT淋巴瘤中HP感染阳性率为80%(32/40)。FISH技术鉴定MALT1基因易位阳性率为7.5%(3/40)。9种免疫细胞浸润水平的对比分析中HP阴性组CD4^(+)T细胞浸润水平显著高于HP阳性组(78.73/HPF vs 40.42/HPF,P<0.001),而MALT1易位阳性组CD56^(+)NK浸润水平(2.76/HPF vs 5.58/HPF,P<0.05)显著低于MALT1易位阴性组。HP阴性组患者的无进展生存率显著低于HP阳性组(P<0.05)。结论HP阴性组中CD4^(+)T细胞浸润水平显著高于HP阳性组,且可能与患者预后相关;MALT1易位阳性组中CD56^(+)NK细胞浸润水平显著低于易位阴性组,提示MALT1易位可能影响以NK细胞为代表的部分先天免疫。Purpose To investigate the effects of helicobacter pylori(HP)infection and MALT1 gene translocation on the immune microenvironment of human gastric mucosa-associated lymphoid tissue(MALT)lymphoma,and to explore the effect on clinicopathological features and prognosis in MALT lymphoma.Methods The infiltration status of CD3^(+)T lymphocytes,CD4^(+)T lymphocytes,CD8^(+)T lymphocytes,Fosp3^(+)regulated T lymphocytes(Treg),CD68^(+)macrophages,CD163^(+)M2 type macrophages,CD83^(+)dendritic cells,CD56^(+)natural killer cells(NK),and CD33^(+)myeloid-derived suppressor cells in 40 cases of MALT lymphoma were detected by immunohistochemistry.HP infection was detected by Hematoxylin and Eosin(HE)staining.The translocation of MALT1 gene was detected by fluorescence in situ hybridization(FISH).The patients were grouped according to HP infection and MALT1 gene translocation and compared the difference of immune microenvironment and patients prognosis.Results The positive rate of HP infection was 80%(32/40),and the HP infection was significantly correlated with infiltration of CD4^(+)T cells(78.73/HPF vs 40.42/HPF,P<0.001)and progression-free survival(P<0.05).The positive rate of MALT1 gene translocation was 7.5%(3/40),which was closely related to the infiltration of CD56^(+)NK cells(2.76/HPF vs 5.58/HPF,P<0.05).Conclusion The level of CD4^(+)T-cell infiltration in HP negative group is significantly higher than that in HP positive group and may be associated with patient outcomes.The level of CD56^(+)NK cell infiltration in the MALT1 translocation positive group is significantly lower than that in the translocation negative group,suggesting that MALT1 translocation may affect part of the innate immunity represented by NK cells.

关 键 词：胃肿瘤 MALT淋巴瘤 免疫微环境 幽门螺旋杆菌 MALT1基因易位 

分 类 号：R733.4[医药卫生—肿瘤]