微小RNA-335-5p靶向轴突导向因子3B对子宫内膜癌细胞增殖、凋亡的影响  

Effects of miR-335-5p targeting SEMA3B on proliferation and apoptosis of endometrial cancer cells

作　　者：王秋菊 郑丽雅[1] 申岩 王园园[1] WANG Qiuju;ZHENG Liya;SHEN Yan;WANG Yuanyuan(Baoding No.1 Central Hospital,Baoding 071000,China;不详)

机构地区：[1]保定市第一中心医院,河北保定071000 [2]保定市安国市医院,河北保定071200

出　　处：《陕西医学杂志》2023年第7期798-802,共5页Shaanxi Medical Journal

基　　金：河北省医学科学研究计划项目(20220315);河北省保定市科技计划项目(2141ZF252)。

摘　　要：目的:探讨微小RNA-335-5p(miR-335-5p)靶向轴突导向因子3B(SEMA3B)对子宫内膜癌细胞(Ishikawa细胞)增殖、凋亡的影响及其作用机制。方法:细胞转染分为miR-335-5p NC组、miR-335-5p inhibitor组、pcDNA组、pcDNA-SEMA3B组、miR-335-5p NC+si NC组、miR-335-5p inhibitor+si NC组、miR-335-5p NC+si SEMA3B组、miR-335-5p inhibitor+si SEMA3B组,未转染的Ishikawa细胞作为对照组。生物信息学预测和双荧光素酶法验证miR-335-5p和SEMA3B的靶向关系;实时荧光定量聚合酶链式反应(qRT-PCR)检测细胞中miR-335-5p和SEMA3B表达;MTT法检测细胞增殖;流式细胞术检测细胞凋亡;蛋白质免疫印迹(WB)检测SEMA3B蛋白表达。结果:miR-335-5p和SEMA3B存在靶向关系,并且在子宫内膜癌组织和Ishikawa细胞中检测到miR-335-5p的高表达和SEMA3B的低表达。与miR-335-5p NC组比较,miR-335-5p inhibitor组细胞增殖率显著降低、细胞凋亡率显著升高(均P<0.05)。与pcDNA组比较,pcDNA-SEMA3B组细胞增殖率显著降低、细胞凋亡率显著升高(均P<0.05)。与miR-335-5p NC+si NC组比较,miR-335-5p inhibitor+si NC组细胞增殖率显著降低、细胞凋亡率显著升高(均P<0.05),miR-335-5p NC+si SEMA3B组细胞增殖率显著升高、细胞凋亡率降低(均P<0.05)。与miR-335-5p NC+si SEMA3B比较,miR-335-5p inhibitor+si SEMA3B组细胞增殖率降低、细胞凋亡率升高(均P<0.05)。与miR-335-5p inhibitor+si NC组比较,miR-335-5p inhibitor+si SEMA3B组细胞增殖率升高、细胞凋亡率降低(均P<0.05)。结论:抑制miR-335-5p可靶向激活SEMA3B表达,从而促进子宫内膜癌细胞的凋亡、抑制癌细胞增殖。Objective:To investigate the effects of miR-335-5p targeting semaphorins 3B(SEMA3B)on the proliferation and apoptosis of endometrial cancer cells(Ishikawa cells)and its mechanism.Methods:Cells were transfected and divided into miR-335-5p NC group,miR-335-5p inhibitor group,pcDNA group,pcDNA-SEMA3B group,miR-335-5p NC+si NC group,miR-335-5p inhibitor+si NC group,miR-335-5p NC+si SEMA3B group,miR-335-5p inhibitor+si SEMA3B group,and the untransfected Ishikawa cells served as the control group.Bioinformatics prediction and dual luciferase method were used to verify the targeting relationship between miR-335-5p and SEMA3B.The qRT-PCR was used to detect the expression of miR-335-5p and SEMA3B in cells.MTT method was used to detect cell proliferatio.Flow cytometry was used to detect cell apoptosis.Western blot(WB)was used to detect the expression of SEMA3B protein.Results:There was a targeting relationship between miR-335-5p and SEMA3B,and high expression of miR-335-5p and low expression of SEMA3B were detected in endometrial cancer tissues and Ishikawa cells.Compared with the miR-335-5p NC group,the cell proliferation rate of the miR-335-5p inhibitor group was significantly reduced,and the cell apoptosis rate was significantly increased(all P<0.05).Compared with the pcDNA group,the cell proliferation rate of the pcDNA-SEMA3B group was significantly reduced,and the cell apoptosis rate was significantly increased(all P<0.05).Compared with miR-335-5p NC+si NC group,the cell proliferation rate of miR-335-5p inhibitor+si NC group was significantly reduced,and the apoptosis rate was significantly increased(all P<0.05),the cell proliferation rate in the miR-335-5p NC+si SEMA3B group was significantly increased,and the cell apoptosis rate was decreased(all P<0.05).Compared with the miR-335-5p NC+si SEMA3B group,the cell proliferation rate in the miR-335-5p inhibitor+si SEMA3B group was decreased,and the apoptosis rate was increased(all P<0.05).Compared with the miR-335-5p inhibitor+si NC group,the cell proliferation rate in t

关 键 词：微小RNA-335-5p 轴突导向因子3B 子宫内膜癌 细胞增殖 细胞凋亡 靶点 

分 类 号：R742[医药卫生—神经病学与精神病学]