三七总皂苷对短暂性前脑缺血大鼠海马区神经元的修复作用实验研究  

Repair effect of panax notoginseng total saponins on hippocampal neurons in rats with transient forebrain ischemia

作　　者：杨涛[1] 刘勇[1] 曹兴华[1] 梁艳山 柯雪茹[1] 陈杰 马晓媛 YANG Tao;LIU Yong;CAO Xinghua;LIANG Yanshan;KE Xueru;CHEN Jie;MA Xiaoyuan(Department of Anesthesiology,Affiliated Hospital of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi 830000,China)

机构地区：[1]新疆医科大学附属中医医院手术麻醉科,新疆乌鲁木齐830000

出　　处：《陕西医学杂志》2023年第7期803-808,共6页Shaanxi Medical Journal

基　　金：新疆维吾尔自治区自然科学基金资助项目(2022D01C546);省部共建中亚高发病成因与防治国家重点实验室开放专项课题(SKL-HIDCA-2020-ZY5)。

摘　　要：目的:探讨三七总皂苷对短暂性前脑缺血大鼠海马区神经元的修复作用。方法:选择30只SD大鼠,随机分为三七总皂苷组、模型组、假手术组,每组10只。模型组、三七总皂苷组使用四血管闭塞建立短暂性前脑缺血大鼠动物模型。假手术组手术方式同三七总皂苷组,不做卡环夹闭、电灼永久性闭塞,仅将右侧颈总动脉、颈内动脉、颈外动脉暴露,之后逐层缝合。三七总皂苷组造模后灌胃给予50 mg/kg三七总皂苷,每天2次,每次间隔12 h,模型组、假手术组灌胃给予等量的0.5%羟甲基纤维素钠。对比三组干预后7、14、28 d的海马神经细胞凋亡、新生神经元数量,对比三组大鼠的学习记忆能力,对比三组干预后7、14、28 d时测量大鼠脑梗死体积及含水量,对比三组DCX/NeuN染色阳性的细胞数量。结果:模型组干预后7、14、28 d的海马神经细胞凋亡明显较三七总皂苷组、假手术组高(均P<0.05);三七总皂苷组干预后7、14、28 d的海马神经细胞凋亡明显较假手术组高(均P<0.05);三七总皂苷组干预后7、14、28 d的海马新生神经元明显较模型组、假手术组高(均P<0.05);干预后7、14、28 d的模型组新生神经元明显较假手术组高(均P<0.05);三七总皂苷组中,随着干预时间延长,海马神经细胞凋亡明显降低,新生神经元明显升高(均P<0.05)。模型组大鼠的潜伏期、错误次数、第1记忆错误次数、第1天学习错误次数、第5天记忆错误次数、第5天学习错误次数明显较三七总皂苷组、假手术组高(均P<0.05),三七总皂苷组大鼠的潜伏期、错误次数、第1天记忆错误次数、第1天学习错误次数、第5天记忆错误次数、第5天学习错误次数明显较假手术组高(均P<0.05)。三七总皂苷组中,随着干预时间延长,第1天记忆错误次数、第1天学习错误次数、第5天记忆错误次数、第5天学习错误次数明显降低(均P<0.05)。模型组的脑梗死体积、Objective:To investigate the repair effect of panax notoginseng total saponins on hippocampal neurons in rats with transient forebrain ischemia.Methods:A total of 30 SD rats were randomly divided into panax notoginseng total saponins group,model group and sham operation group,with 10 rats in each group.Model group and panax notoginseng total saponin group were treated with four-vessel occlusion to establish the rat model of transient forebrain ischemia.The operation method of the sham operation group was the same as that of the panax notoginseng total saponins group,and the right common carotid artery,internal carotid artery and external carotid artery were exposed and sutured layer by layer instead of clamping or permanent occlusion by electrocauterization.Panax notoginseng total saponins group was given 50 mg/kg panax notoginseng total saponins by intragastric administration,twice a day at an interval of 12 hours.Model group and sham operation group were given the same amount of 0.5%hydroxymethylcellulose sodium by intragastric administration.Hippocampal nerve cell apoptosis and the number of new neurons in the three groups were compared at 7,14 and 28 days after intervention.The learning and memory ability of the three groups were compared.The volume and water content of cerebral infarction in the three groups were compared at 7,14 and 28 days after intervention.The number of DCX/NeuN staining positive cells in the three groups were compared.Results:The apoptosis of hippocampal nerve cells in model group was significantly higher than that in panax notoginseng total saponins group and sham operation group at 7,14 and 28 days after intervention(all P<0.05).The hippocampal nerve cell apoptosis of panax notoginseng total saponins group was significantly higher at 7,14 and 28 days after intervention than that of sham operation group(all P<0.05).The number of hippocampal new neurons in panax notoginseng total saponins group was significantly higher at 7,14 and 28 days after intervention than that in model and sham o

关 键 词：三七总皂苷 短暂性前脑缺血 海马 神经元 修复 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]