miR599靶向特异AT序列结合蛋白2对非小细胞肺癌A549细胞迁移和侵袭能力的影响  

Effects of miR599 targeting specific AT sequence binding protein 2 on migration and invasion of non-small cell lung cancer A549 cells

作　　者：占志强[1] 黄行志 李婷 罗湘[1] 温莹浩[1] 彭辉 罗锋珩 ZHAN Zhiqiang;HUANG Xingzhi;LI Ting;LUO Xiang;WEN Yinghao;PENG Hui;LUO Fengheng(Department of Oncology,Pingxiang People's Hospital,Pingxiang,Jiangxi,337055,China)

机构地区：[1]萍乡市人民医院肿瘤科,江西萍乡337055

出　　处：《当代医学》2022年第29期29-31,共3页Contemporary Medicine

基　　金：江西省卫生健康委科技计划项目(202140501)。

摘　　要：目的探讨miR599靶向特异AT序列结合蛋白2对非小细胞肺癌A549细胞迁移和侵袭能力的影响。方法取冻存A549细胞复苏、培养,取对数生长期细胞用于实验。随机将对数生长期A549细胞分为对照组、miR-599 mimics组、miR-599 inhibitor组、miR-599 mimics联合pGV141-SATB2组,分别转染空白试剂、miR-599 mimics、miR-599 inhibitor和miR-599 mimics联合pGV141-SATB2。采用qPCR法检测转染前后A549细胞中miR-599 mRNA表达水平,采用Transwell小室检测法测定A549细胞迁移和侵袭能力,采用Western blot法测定细胞SATB2、E-cadherin、N-cadherin、vimentin蛋白表达水平。结果对照组、miR-599 mimics组、miR-599 inhibitor组和miR-599 mimics联合pGV141-SATB2组A549细胞miR-599 mRNA表达水平比较差异有统计学意义(P<0.05)。对照组、miR-599 mimics组、miR-599 inhibitor组和miR-599 mimics联合pGV141-SATB2组迁移和侵袭细胞数量比较差异有统计学意义(P<0.05)。上述4组SATB2、E-cadherin、N-cadherin、vimentin蛋白表达水平比较差异有统计学意义(P<0.05)。结论miR-599可通过负调控SATB2,促进上皮间质转化,增强A549细胞迁移和侵袭能力。Objective To investigate the effects of miR-599 targeting specific AT sequence binding protein 2 on the migration and invasion of non-small cell lung cancer(NSCLC)A549 cells.Methods Frozen storage A549 cells were resuscitated and cultured,and logarithmic growth cells were taken for experiment.A549 cells in logarithmic growth phase were randomly divided into control group,miR-599 mimics group,miR-599 inhibitor group,miR-599 mimics combined with PGV141-SATB2 group.The blank reagent,miR-599 mimics,miR-599 inhibitor,and miR-599 mimics combined with PGV141-SATB2 were transfected respectively.The expression level of miR-599 mRNA in A549 cells before and after transfection was detected by qPCR method,and the migration and invasion ability of A549 cells were determined by Transwell chamber test.The protein expression levels of SATB2,E-cadherin,N-cadherin and vimentin were determined by Western blot.Results There were statistically significant differences in miR-599 mRNA expression levels in A549 cells among the control group,miR-599 mimics group,miR-599 inhibitor group and miR-599 mimics combined with PGV141-SATB2 group(P<0.05).There were statistically significant differences in the number of migrated and invaded cells among the control group,miR-599 mimics group,miR-599 inhibitor group and miR-599 mimics combined with PGV141-SATB2 group(P<0.05).There were statistically significant differences in the protein expression levels of SATB2,E-cadherin,N-cadherin and vimentin among the above 4 groups(P<0.05).Conclusion MiR-599 can negatively regulate SATB2,promote epithelial mesenchymal transformation,and enhance the migration and invasion ability of A549 cells.

关 键 词：miR599 特异AT序列结合蛋白2 非小细胞肺癌 A549细胞 

分 类 号：R734.2[医药卫生—肿瘤]