医学院校环境生物气溶胶分布特征研究  

Study on the distribution characteristics of bioaerosols in the medical college environment

作　　者：章敏[1] 萧闵[1] 田代志[1] ZHANG Min;XIAO Min;TIAN Daizhi(Hubei University of Chinese Medicine,Hubei,Wuhan 430065,China)

机构地区：[1]湖北中医药大学,湖北武汉430065

出　　处：《中国医药科学》2023年第12期179-182,197,共5页China Medicine And Pharmacy

基　　金：湖北省科技创新基地条件平台专项(2021DFE028)。

摘　　要：目的生物气溶胶近年受到广泛关注,本研究拟对湖北中医药大学校园内生物气溶胶以及气载抗性基因的分布情况进行本底调查及健康风险评估,为中医药相关生物气溶胶的研究提供新的参考。方法采用自研生物气溶胶收集器,对中医院校中5个典型环境中的生物气溶胶收集后进行高通量测序,进而对微生物的分布特征,多样性、相关性进行分析,了解对应环境中气溶胶微生物的种类以及相对丰度,分析种属相关性。在此基础上,对气载抗性基因进行检测,统计不同环境中的阳性率。结果研究区间采集样本显示,在门分类水平上,中医院校内优势菌群分别为变形菌门(proteobacteria)、放线菌门(actinobacteria)、厚壁菌门(firmicutes)和拟杆菌门(bacteroidetes),不同样点优势菌群存在统计差异。寡养单胞菌属(stenotrophomonas)丰度最高,各样点优势菌属及含量差异显著。同期抗性基因检测结果显示,四环素抗性基因与磺胺类抗性基因为样本环境中主要抗性基因。结论实验室中气载抗性基因具有最高的多样性,可能与日常实验中干预药物的广泛使用有关。Objective Bioaerosols have received extensive attention in recent years.This study intends to conduct a background investigation and health risk assessment on the distribution of bioaerosols and airborne resistance genes on the campus of the Hubei University of Chinese Medicine,to provide a new reference for the study of bioaerosols related to traditional Chinese medicine.Methods Self-developed bioaerosol collectors were used to collect bioaerosols from five typical environments in the Chinese medicine college,and then highthroughput sequencing was carried out to analyze the distribution characteristics,diversity,and correlation of microorganisms,understand the species and relative abundance of aerosol microorganisms in the corresponding environment,and analyze the species correlation.On this basis,airborne resistance genes were tested and the positive rates in different environments were counted.Results According to the sample collection within the study interval,at the phylum classification level,the dominant bacterial communities within the Chinese medicine college were Proteobacteria,Actinobacteria,Firmicutes,and Bacteroidetes,respectively.There were statistical differences in the dominant bacterial communities at different sample points.The abundance of Stenotrophomonas was the highest,and there were significant differences in the dominant bacterial genus and content among different sample points.The results of simultaneous resistance gene testing showed that tetracycline resistance genes and sulfonamide resistance genes were the main resistance genes in the sample environment.Conclusion Airborne resistance genes have the highest diversity in the laboratory,which may be related to the widespread use of intervention drugs in daily ex periments.

关 键 词：中医院校 生物气溶胶 抗性基因 高通量测序 

分 类 号：R122.1[医药卫生—环境卫生学]