HPV介导DNA甲基化对口咽癌患者预后的影响  

Role of Activation of DNA Methylation via HPV in Prognosis of Oropharyngeal Carcinoma

作　　者：孙纪威 陈广进 唐清明[1,2] 张珺媛 张曼 郭丰源 杨成[1,2] Sun Jiwei;Chen Guangjin;Tang Qingming(Department of Stomatology,Union Hospital,Tongji Medical College,Hua zhong University of Science and Technology,Hubei Province Key Laboratory of Oral and Maxillofacial Develo pment and Regeneration,Wuhan 430022,China;School of Stomatology,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China)

机构地区：[1]华中科技大学同济医学院附属协和医院口腔科,口腔颌面发育与再生湖北省重点实验室,武汉430022 [2]华中科技大学同济医学院口腔医学院,武汉430030

出　　处：《华中科技大学学报（医学版）》2023年第3期283-291,共9页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金青年基金资助项目(No.81800986);湖北省重点研发计划项目(No.2020BCA072);中国科协青年人才托举工程项目(No.2018QNRC001)。

摘　　要：目的利用生物信息学方法探讨HPV在口咽癌发生发展中的作用及其机制。方法将甲基化微阵列数据及转录组数据结合患者临床信息联合分析,解析口咽癌中HPV诱导的甲基化驱动基因及其生物学功能。结果富集分析显示HPV^(+)口咽癌患者的免疫活动明显激活,抗肿瘤T细胞免疫功能尤为活跃,这可能为HPV^(+)口咽癌患者更好的临床预后提供了一个合理的解释;CCR7和CXCR3可能是具有重大预后价值的HPV相关甲基化驱动基因。结论本研究揭示了HPV诱导的新型抗肿瘤免疫机制,为HPV^(+)与HPV^(-)口咽癌患者的预后差异提供了可能的依据,并提出HPV相关甲基化驱动基因可能是预测口咽癌患者临床预后的有效指标。Objective To explore the role of human pa pillomavirus(HPV)in the development of oropharyngeal cancer,and its mechanisms.Methods In this study,we analyzed methylation microarray data and transcriptomic sequencing data in con-junction with clinical information to decipher the methylation-driven genes induced by HPV and their biological functions in oro-pharyngeal carcinoma.Results Enrichment analysis showed that immune activity in HPV^(+)patients was significantly activated,with particularly active anti-tumor T cell immune function,providing a reasona ble explanation for the better clinical prognosis of HPV^(+)oropharyngeal cancer patients.CCR7 and CXCR3 were identified as HPV^(-)related methylation-driven genes with signifi-cant prognostic value.Conclusion This study revealed a novel anti-tumor immune function induced by HPV,providing possible evidence for the differential clinical outcomes of HPV^(+)oropharyngeal carcinoma patients,and suggesting that HPV^(-)related methylation-driven genes may serve as effective indicators for predicting clinical prognosis in oropharyngeal carcinoma patients.

关 键 词：口咽癌 甲基化 TCGA数据库 CCR7 CXCR3 

分 类 号：R739.8[医药卫生—肿瘤]