江苏宿迁地区长链非编码RNA H19基因多态性与胃癌易感性的关联分析  

Study on relationship between long chain non-coding RNA H19 genetic polymorphisms and susceptibility to gastric cancer in Suqian area of Jiangsu

作　　者：邱巍 陈素梅 刘东声 QIU Wei;CHEN Sumei;LIU Dongsheng(Department of Clinical Laboratory,Affiliated Suqian Hospital,Xuzhou Medical University/Suqian Municipal Key Laboratory of Laboratory Medicine,Suqian,Jiangsu 223800,China)

机构地区：[1]徐州医科大学附属宿迁医院检验科/江苏省宿迁市检验医学重点实验室,江苏宿迁223800

出　　处：《检验医学与临床》2023年第13期1914-1918,共5页Laboratory Medicine and Clinic

基　　金：江苏省宿迁市科技计划项目(S201813);江苏省宿迁市检验医学重点实验室项目(M201603)。

摘　　要：目的探讨江苏宿迁地区长链非编码RNA H19(LncRNA H19)基因多态性与胃癌易感性的关系。方法选取2019年1月至2021年12月该院收治的265例胃癌患者(胃癌组)和284例健康体检者(对照组)作为研究对象。提取其外周血基因组DNA,运用限制性片段长度多态性PCR技术检测两组研究对象LncRNA H19 rs217727 C>T和rs2839698 C>T位点的基因型、等位基因分布频率。结果对照组rs217727 C>T位点CC、CT、TT基因型频率分别为39.1%、48.6%、12.3%;胃癌组rs217727 C>T位点CC、CT、TT基因型频率分别为30.9%、50.2%、18.9%。两组CC、TT基因型频率比较,差异有统计学意义(P<0.05)。TT纯合子发生胃癌的风险是CC的1.93倍(OR=1.93,95%CI=1.15~3.25),在年龄≥60岁的男性胃癌患者中TT基因型有较高的风险。对照组和胃癌组rs2839698 C>T位点CC、CT、TT基因型频率分别为57.4%、35.6%、7.0%和49.8%、38.5%、11.7%。两组CC、TT基因型频率比较,差异有统计学意义(P<0.05),TT纯合子发生胃癌的风险是CC的1.91倍(OR=1.91,95%CI=1.04~3.51),在年龄≥60岁的男性胃癌患者中TT基因型有较高的风险。结论LncRNA H19 rs217727 C>T和rs2839698 C>T位点与胃癌的遗传易感性有关联,可作为胃癌遗传易感性升高的危险因素。Objective To investigate the relationship between long chain non-coding RNA H19(LncRNA H19)gene polymorphisms and susceptibility to gastric cancer.Methods A total of 265 patients with gastric cancer(gastric cancer group)treated in this hospital and 284 healthy subjects undergoing physical examination(control group)were selected as the study subjects.The peripheral blood genomic DNA was extracted.Polymerase chain reaction with restriction fragment length polymorphism was employed to detect the genotypes and allele distribution frequencies of LncRNA H19 rs217727 C>T and rs2839698 C>T in the study subjects of the two groups.Results The genotype frequencies of genotypes CC/CT/TT at rs217727 C>T site in the control group were 39.1%,48.6%and 12.3%respectively;and which in the gastric cancer group were 30.9%,50.2%and 18.9%respectively.The frequency of CC/TT genotypes had statistical difference between the control group and gastric cancer group(P<0.05).The gastric cancer occurrence risk in TT homozygotes was 1.93 fold of CC(OR=1.93,95%CI=1.15-3.25).The TT genotype had more risk among the male patients with gastric cancer aged≥60 years old.The genotype frequencies of CC/CT/TT at rs2839698 C>T site in the control group were 57.4%,35.6%and 7.0%in the control group and 49.8%,38.5%and 11.7%in the gastric cancer group,respectively.The CC and TT genotype frequency had statistical difference between the two groups(P<0.05).The risk of gastric cancer occurrence in the TT homozygotes was 1.91 fold of CC(OR=1.91,95%CI=1.04-3.51).The TT genotype had the higher risk among the male patients with gastric cancers aged≥60 years old.Conclusion The LncRNA H19 at rs217727 C>T and rs2839698 C>T loci is related with genetic susceptibility to gastric cancer,which could serve as the risk factor for increased genetic susceptibility to gastric cancer.

关 键 词：长链非编码RNA H19 胃癌 单核苷酸多态性 

分 类 号：R735.2[医药卫生—肿瘤]