Highly efficient fermentation of glycerol and 1,3-propanediol using a novel starch as feedstock  

作　　者：You Yang Ying Chen Yanling Jin Jing Liu Xiang Qin Wenjun Liu Ling Guo 

机构地区：[1]Department of Pediatrics,Children Hematological Oncology and Birth Defects Laboratory,The Affiliated Hospital of Southwest Medical University,Sichuan Clinical Research Center for Birth Defects,Luzhou,Sichuan,646000,China [2]CAS Key Laboratory of Environmental and Applied Microbiology,Environmental Microbiology Key Laboratory of Sichuan Province,Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu,Sichuan,610041,China [3]National Engineering Research Center for Non-Food Biorefinery,Guangxi Academy of Sciences,Nanning,Guangxi,530007,China

出　　处：《Food Bioscience》2022年第2期200-208,共9页食品生物科学（英文）

基　　金：This work was funded by Medical Science and Technology Project of Health Commission of Sichuan Province(No.21PJ091);the Special Project of Science and Technology Research of Sichuan Administration of Traditional Chinese Medicine(2020JC0135);the Applied Basic Research Project of Southwest Medical University(2021ZKQN083);the Doctoral Research Initiation Fund of the Affiliated Hospital of Southwest Medical University;The Basic Research Project of Sichuan Province(No.2019YJ0690);The Major Science and Technology Projects in Sichuan Province(No.2019YFS0531);the University-level Scientific Research Project of Southwest Medical University(2020ZRQNB029);and Natural Science Foundation of Guangxi,China(2020GXNSFAA259021).

摘　　要：As a potential cost-effective feedstock for highly efficient fermentation of glycerol and its downstream product 1,3-propanediol(1,3-PD),duckweed starch was characterized and used for glycerol fermentation,for the first time,in this study.Genes involved in glycerol biosynthesis(gpd1 and gpp2)were overexpressed in Escherichia coli,and genes involved in glycerol catabolism(glpK and gldA)were disrupted,which led to significantly decreased residual sugar levels and dramatically increased glycerol production.The maximum glycerol concentration in fed-batch fermentation reached 102.72 g L^(-1) at 28 h,and the glycerol productivity was 3.67 g L^(-1) h^(-1),which,to our knowledge,is the highest productivity thus far reported.Subsequently,glycerol broth was fermented into 1,3-PD by Klebsiella pneumoniae.The concentration,conversion rate and productivity of 1,3-PD reached 35.54 g L^(-1),40.28%and 0.89 g L^(-1) h^(-1),respectively,without optimization.In summary,the duckweed starch-to-glycerol-to-1,3-PD process is feasible and shows potential for improving glycerol industry competitiveness.

关 键 词：Duckweed starch GLYCEROL 1 3-PROPANEDIOL Fed-batch fermentation BIOSYNTHESIS 

分 类 号：TQ923[轻工技术与工程—发酵工程]