Linkages of volatile fatty acids and polyhexamethylene guanidine stress during sludge fermentation:Metagenomic insights of microbial metabolic traits and adaptation  

作　　者：Feng Wang Wei Du Wenxuan Huang Shiyu Fang Xiaoshi Cheng Leiyu Feng Jiashun Cao Jingyang Luo Yang Wu 

机构地区：[1]Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes,Ministry of Education,Hohai University,Nanjing 210098,China [2]College of Environment,Hohai University,Nanjing 210098,China [3]State Key Laboratory of Pollution Control and Resource Reuse,School of Environmental Science and Engineering,Tongji University,Shanghai 200092,China

出　　处：《Chinese Chemical Letters》2023年第6期172-177,共6页中国化学快报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(No.52070069);Jiangsu Province Natural Science Foundation(No.BK20211207);China Postdoctoral Science Foundation(No.2021M692423);Shanghai Post-doctoral Excellence Program(No.2020419);State Key Laboratory of Pollution Control and Resource Reuse Foundation(No.PCRRF20005);the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),China。

摘　　要：The massive use of polyhexamethylene guanidine(PHMG),as a typical bactericidal agent,raised environmental concerns to the public.This work comprehensively revealed the hormesis effects of PHMG occurred in waste activated sludge(WAS)on the generation of volatile fatty acids(VFAs)during anaerobic fermentation.The low level of PHMG(100 mg/g TSS)significantly promoted the VFAs generation(1283 mg COD/L,compared with 337 mg COD/L in the control)via synchronously facilitating the solubilization,hydrolysis,and acidification steps but inhibiting methanogenesis.Metagenomic analysis showed that the functional anaerobe(i.e.,Bacteroides,Macellibacteroide and Parabacteroide)and corresponding genetic expressions responsible for extracellular hydrolysis(i.e.,clp P),membrane transport(i.e.,ffh and gsp F),intracellular substrates metabolism(i.e.,ald and paa F)and VFAs biosynthesis(i.e.,ACACA and FASN)were enhanced in the optimal presence of PHMG.Moreover,the anaerobic species could respond and adapt to low PHMG stimuli via quorum sensing(i.e.,cqs A,rpf C and rpf G),and thus maintain the high microbial metabolic activities.However,they were unable to tolerate the toxicity of excessive PHMG,resulting in the extremely low VFAs production.This work enlightened the effects of emerging pollutants on WAS fermentation at the genetic levels,and provided guidance on the WAS treatment and resource recovery.

关 键 词：Polyhexamethylene guanidine(PHMG) Waste activated sludge(WAS) Volatile fatty acids(VFAs) Metabolic pathways Adaptive mechanisms 

分 类 号：X703[环境科学与工程—环境工程] TQ920.1[轻工技术与工程—发酵工程]