分离自母婴肠道的假小链双歧杆菌的耐药性分析  

Analysis of antibiotic resistance of Bifidobacterium pseudocatenulatum isolated from the guts of mothers and infants

作　　者：马昕玮 沈婷婷 路镜达 郭圆圆 朱梦缘 钟智[1] MA Xinwei;SHEN Tingting;LU Jingda;GUO Yuanyuan;ZHU Mengyuan;ZHONG Zhi(Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education,Key Laboratory of Dairy Products Processing,Ministry of Agriculture and Rural Affairs,Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,Inner Mongolia,China)

机构地区：[1]内蒙古农业大学乳品生物技术与工程教育部重点实验室、农业农村部奶制品加工重点实验室、内蒙古乳品生物技术与工程重点实验室,内蒙古呼和浩特010018

出　　处：《微生物学通报》2023年第6期2349-2362,共14页Microbiology China

基　　金：内蒙古自治区科技计划(2021GG0080)。

摘　　要：【背景】由于滥用抗生素导致细菌耐药性日益严重。对于双歧杆菌,人们往往注重其益生功能的挖掘而忽视了对其耐药性的研究,存在一定的安全隐患。【目的】检测母婴肠道中假小链双歧杆菌的耐药性,探究婴儿肠道中假小链双歧杆菌耐药性的来源。【方法】利用微量肉汤稀释法测定48株分离自母婴肠道的假小链双歧杆菌对14种抗生素的耐药性,比较分离自不同家庭母婴肠道中假小链双歧杆菌的耐药性。【结果】48株母婴肠道分离株对四环素、氯霉素、新霉素、环丙沙星100%耐药,对其余10种抗生素耐药率依次为:卡那霉素98%、利福平80%、克林霉素78%、甲氧苄啶63%、红霉素59%、庆大霉素43%、链霉素16%、万古霉素14%、氨苄西林6%、利奈唑胺2%。母婴肠道分离株的耐药性无显著差异,分离自同一家庭母婴肠道的菌株具有相似的耐药表型。【结论】分离自母婴肠道的假小链双歧杆菌对多种抗生素具有耐药性,婴儿肠道中假小链双歧杆菌的耐药性可能是由母亲肠道垂直传递而来。[Background]Caused by the abuse of antibiotics,the rapid emergence of resistant bacteria is occurring worldwide.For Bifidobacterium,the probiotic property has been emphasized,while there is a paucity of research on the antibiotic resistance.[Objective]To detect the antibiotic resistance of Bifidobacterium pseudocatenulatum isolated from the feces of mothers and infants and to explore the source of resistant strains in infant gut.[Methods]The resistance of 48 strains of B.pseudocatenulatum isolated from the feces of mothers and infants to 14 antibiotics was determined by the minimum inhibitory concentration method,and the resistance of strains isolated from different families was compared.[Results]All the 48 strains were resistant to tetracycline,chloramphenicol,neomycin,and ciprofloxacin,and 98%,80%,78%,63%,59%,43%,16%,14%,6%,and 2% of the strains were resistant to kanamycin,rifamycin,clindamycin,trimethoprim,erythromycin,gentamicin,streptomycin,vancomycin,ampicillin,and linezolid,respectively.There were no significant differences in antibiotic resistance between the isolates from mothers and infants,and isolates from the same family had similar antibiotic-resistant phenotypes.[Conclusion]The B.pseudocatenulatum isolated from feces of mothers and infants were resistant to a variety of antibiotics.The resistant strains in infant gut may be vertically transmitted from the mother՚s gut.

关 键 词：假小链双歧杆菌 母婴肠道 耐药性 最小抑菌浓度 

分 类 号：R446.5[医药卫生—诊断学]