牡丹江地区2015—2021年食源性疾病监测及沙门菌同源性调查  

Food borne disease surveillance and Salmonella homology investigation in Mudanjiang,2015-2021

作　　者：高艳君 沙丽娜[2] 陈嘉璐 GAO Yanjun;SHA Lina;CHEN Jialu(Food Department of Mudanjiang Comprehensive Health Supervision and Law Enforcement Bureau,Mudanjiang,Heilongjiang 157000,China;不详)

机构地区：[1]牡丹江市卫生健康综合监督执法局食品科,黑龙江牡丹江157000 [2]牡丹江市疾病预防控制中心

出　　处：《中国国境卫生检疫杂志》2023年第3期237-240,共4页Chinese Journal of Frontier Health and Quarantine

基　　金：牡丹江市应用技术研究与开发计划项目(HT2020NS029)。

摘　　要：目的调查牡丹江地区食源性疾病流行情况及沙门菌分型,为制定食源性疾病防控措施提供参考。方法收集牡丹江地区2015—2021年1256例食源性疾病患者粪便或肛拭子标本,采用全自动生化系统鉴定致泻性大肠埃希菌、空肠弯曲菌、副溶血性弧菌、沙门菌、嗜水气单胞菌、志贺菌、诺如病毒等病原体,分析沙门菌血清学分型和分子生物学分型。结果共收集1256例食源性疾病患者样本1084份,病原体检出率为11.62%(126/1084)。不同性别食源性疾病患者的病原体检出率差异无统计学意义(P>0.05),不同年龄、发病季节的食源性疾病患者病原体检出率差异均有统计学意义(P<0.05),以0~6岁多发,发病季节主要为夏季;混合类和水产类食品的病原体检出率分别为20.56%和16.40%,高于其他种类食品(P<0.05);126株病原体中沙门菌最多,占26.98%(34/126),其次为致泻性大肠埃希菌,占19.05%(24/126);分型的32株沙门菌可分为9种血清型,主要为肠炎沙门菌和鼠伤寒沙门菌,分别占41.18%和23.53%;27株成功分子生物学分型,PFGE指纹图谱带型相似度为44%~100%,8株鼠伤寒沙门菌聚集为2簇,2簇相似度均接近100%,2株布里丹沙门菌高度同源,带型相似度接近100%。结论牡丹江地区2015—2021年食源性疾病患者主要为6岁以下儿童,夏季高发,混合类和水产类为主要致病食品,主要病原体为沙门菌。Objective To investigate the prevalence of food borne diseases in Mudanjiang,to analyze the types of Salmonella,and provide reference for the formulation of food borne disease prevention and control measures.Methods From 2015 to 2021,feces or anal swabs of patients with food borne diseases in Mudanjiang were collected,the automatic biochemical system was used to identify the pathogens including diarrhoeal Escherichia coli,Campylobacter jejuni,Vibrio parahaemolyticus,Salmonella,Aeromonas hydrophila,Shigella and Norovirus.The serotype and molecular biological type of Salmonella were analyzed.Results A total of 1084 samples were collected from 1256 patients with food borne diseases,and the total positive rate of pathogens was 11.62%(126/1084).There was no statistically significant difference in the positive rate of pathogens among patients with food borne diseases of different genders(P<0.05)and there was significant difference in the positive rate of pathogens among different ages and onset seasons patients(P<0.05).It occured frequently at the age of 0-6,and the onset season was mainly summer.The pathogens were detected positively from mixed food and aquatic products with the positive rate of 20.56%and 16.40%,respectively,which were higher than other kinds of food(P<0.05).Among 126 pathogens,Salmonella was dominant,accounted for 26.98%(34/126),followed by diarrhea Escherichia coli,accounted for 19.05%(24/126).Among 34Salmonella strains,32 strains were classified into 9 serotypes,mainly were Salmonella enteritidis and Salmonella typhimurium,accounted for 41.18%and 23.53%,respectively.27 strains were successfully typed with the similarity of PFGE fingerprint bands of 44%-100%,8 Salmonella typhimurium strains were clustered into 2 clusters,with the similarity close to 100%,the two strains of Salmonella bridan were highly homologous with the similarity near to100%.Conclusion The patients with food borne diseases in Mudanjiang from 2015 to 2021 were mainly children under 6 years old,with a high incidence in summer,mixed

关 键 词：食源性疾病 沙门菌 血清型 生物学分型 

分 类 号：R183.4[医药卫生—流行病学] R155.5[医药卫生—公共卫生与预防医学]