miRNA-24靶向调控MEK/ERK信号通路对乳腺癌转移的作用  

Effect of miRNA-24-Targeted Regulation of MEK/ERK Signaling Pathway on Breast Cancer Metastasis

作　　者：曹琳 尹红[1] 朱静 段甚佳 肖立新[1] CAO Lin;YIN Hong;ZHU Jing;DUAN Shen-jia;XIAO Li-xin(Department of Breast Surgery,Hunan Maternal and Child Health Hospital,Changsha 410000,China)

机构地区：[1]湖南省妇幼保健院乳腺外科,长沙410000

出　　处：《南昌大学学报（医学版）》2023年第3期12-17,共6页Journal of Nanchang University：Medical Sciences

摘　　要：目的探讨miRNA-24(miR-24)靶向调控MEK/ERK信号通路对乳腺癌转移的作用。方法体外培养乳腺癌(BC)细胞系MCF-7、MDA-MB-231细胞以及非肿瘤性人乳房上皮细胞系H184B5F5/M10细胞,采用qPCR方法检测各细胞中miR-24的表达水平。将MCF-7细胞分为正常组、miRNA阴性对照组(miR-24-NC组)和miR-24抑制剂组,采用CCK8、伤口愈合试验和TUNEL检测细胞活力、迁移和凋亡情况,采用蛋白质印迹法检测细胞凋亡相关蛋白和MEK/ERK通路相关蛋白的表达水平。结果miR-24在MCF-7和MDA-MB-231细胞中的表达水平显著高于H184B5F5/M10细胞(P<0.05)。与miR-24-NC组相比,miR-24抑制剂组抑制了细胞的活力和迁移(P<0.05),促进了细胞的凋亡(P<0.05);其凋亡蛋白Bcl-2显著降低(P<0.05),Bax、caspase-3和caspase-9显著升高(P<0.05);同时降低了MEK/ERK信号通路的表达(P<0.05)。结论乳腺癌细胞中miR-24表达升高,并且通过调控MEK/ERK信号通路促进乳腺癌的转移。Objective Purpose To explore the effect of miRNA-24-targeted regulation of MEK/ERK signaling pathway on breast cancer metastasis.Methods The expression of miR-24 was detected by qPCR in BC cell lines MCF-7 and MDA-MB-231 and non-neoplastic human breast epithelial cell line H184B5F5/M10.The MCF-7 cells were divided into normal group,miRNA-negative control group(miR-24-NC group),and miR-24 inhibitor group.Cell viability,migration and apoptosis were measured by CCK8,wound healing test and TUNEL assays,respectively.The expression levels of apoptosis-related proteins and MEK/ERK pathway-related proteins in BC cells were detected by Western blotting.Results The expression of miR-24 in MCF-7 and MDA-MB-231 cells was higher than that in H184B5F5/M10 cells(P<0.05).Compared with the miR-24-NC group,cell viability and migration were suppressed in the miR-24 inhibitor group(P<0.05).Furthermore,the miR-24 inhibitor promoted cell apoptosis with decreased Bcl-2 expression and increased Bax,caspase-3 and caspase-9 expression(P<0.05).In addition,MEK/ERK pathway-related proteins were down-regulated in the miR-24 inhibitor group(P<0.05).Conclusion miR-24 is highly expressed in BC cells,and promotes BC metastasis by regulating MEK/ERK signaling pathway.

关 键 词：miRNA-24 MEK/ERK信号通路 乳腺癌 转移 

分 类 号：R737.9[医药卫生—肿瘤]