铁代谢异常在乙型肝炎病毒感染慢性化中作用机制的初步研究  

A Pilot Study on the Mechanism of Abnormal Iron Metabolism in the Chronic Hepatitis B Virus Infection

作　　者：潘颖 杨凯 孙蓓蓓[2] PAN Ying;YANG Kai;SUN Beibei(Anhui Medical College,Anhui Hefei 230601;The Second Affiliated Hospital Of Anhui Medical University,Hefei,Anhui 230601,China)

机构地区：[1]安徽医学高等专科学校 [2]安徽医科大学第二附属医院,安徽合肥230601

出　　处：《九江学院学报（自然科学版）》2023年第2期95-98,共4页Journal of Jiujiang University：Natural Science Edition

基　　金：安徽省自然科学基金面上项目(编号2108085MH301);安徽省高校自然科学研究重点项目(编号KJ2020A0861)的成果之一。

摘　　要：目的 研究慢性乙型肝炎患者肝组织铁的表达及分布,并在体外细胞初步探讨铁代谢异常在乙肝病毒感染慢性化中的作用机制。方法 以普鲁氏蓝铁染色检测27例不同程度的慢性乙型肝炎(CHB)患者及3例非CHB患者肝组织铁表达情况并分析肝组织中铁的表达与CHB严重程度的关系;比较分析HepG2细胞和含有完整的HBV病毒颗粒的HepG2.2.15细胞内和上清中铁代谢相关指标含量的差异;运用柠檬酸铁处理HepG2.2.15细胞,荧光定量PCR技术检测细胞上清HBV-DNA的水平,化学发光法测定细胞上清液中HBsAg和HBeAg含量。结果 普鲁氏蓝铁染色结果显示,CHB患者肝组织中铁的表达量显著高于非CHB患者肝组织,且与肝脏炎症等级呈显著正相关;HepG2.2.15细胞内及上清液中铁蛋白的含量显著高于HepG2细胞(p<0.01);柠檬酸铁可促进HepG2.2.15分泌HBV-DNA及乙肝抗原的表达。结论 HBV感染可诱导肝组织的铁沉积,引起铁代谢紊乱;而铁可促进HBV的复制及抗原的分泌。Objective To study the expression and distribution of iron in liver tissues in patients with chronic hepatitis B,and the mechanism of abnormal iron metabolism in the chronic hepatitis B virus infection was preliminarily discussed in vitro cells.Method Perl?s Prussian Blue stain was used to detect the iron expression in the liver tissues of 27 patients with different degrees of chronic hepatitis B(CHB) and 3 non-CHB patients,and analyzed the relationship between the iron expression in the liver tissues and the severity of liver inflammation.Analysis of the content of iron metabolism-related indicators in HepG2 cells and HepG2.2.15 cells containing intact HBV virus particles and in the supernatant.HepG2.2.15 cells were treated with ferric citrate,then the level of HBV-DNA in the cell supernatant was detected by fluorescence quantitative PCR,and the secretion of HBsAg and HBeAg in the cell supernatant was determined by electrochemiluminescence.Result Perl?s Prussian Blue stain showed that the expression of iron in liver tissues of CHB patients was significantly higher than that of non-CHB patients,and it was significantly positively correlated with the grade of liver inflammation;the content of ferritin in HepG2.2.15 cells and in the supernatant was significant higher than HepG2 cells(p0.001);ferric citrate promoted the levels of HBV-DNA and the secretion of hepatitis B antigen.Conclusion HBV infection can induce iron deposition in liver tissues and cause iron metabolism disorders;while iron can promote HBV replication and antigen secretion.

关 键 词：乙肝病毒 炎症 铁沉积 铁蛋白 

分 类 号：R575.3[医药卫生—消化系统]