5-氮杂-2’-脱氧胞苷对苯并[a]芘致海马神经元毒性的保护作用  

Protective effects of 5-Aza-2’-deoxycytidine on neurotoxicity of hippocampal neurons induced by benzo[a]pyrene

作　　者：魏智超 冯婧宇 聂继盛[1] 魏建宏[2] WEI Zhichao;FENG Jingyu;NIE Jisheng;WEI Jianhong(School of Public Health,Shanxi Medical University,Shanxi Province,Taiyuan 030000,China;Fenyang College of Shanxi Medical University,Shanxi Province,Fenyang 032200,China)

机构地区：[1]山西医科大学公共卫生学院,山西太原030000 [2]山西医科大学汾阳学院,山西汾阳032200

出　　处：《中国医药导报》2023年第16期26-30,共5页China Medical Herald

基　　金：山西省自然科学基金项目(201801D121264);山西省吕梁市重点研发项目(2021SHFZ-1-50)。

摘　　要：目的探讨5-氮杂-2’-脱氧胞苷(5-Aza-cdR)对苯并[a]芘(B[a]P)致海马神经元毒性的保护作用。方法体外培养海马神经元至第5天,观察并鉴定细胞。实验设立对照组,B[a]P组(20μmol/L B[a]P)和B[a]P+5-Aza-cdR组(20μmol/L B[a]P+10μmol/L 5-Aza-cdR)。观察细胞形态;检测细胞活力、DNA甲基转移酶蛋白和基因表达水平;测定细胞中的总甲基化水平和细胞凋亡情况。结果与对照组比较,B[a]P组海马神经元活性降低(P<0.05);细胞凋亡率升高(P<0.05);DNMT3A、DNMT3B基因和DNMT1、DNMT3A蛋白表达水平上升(P<0.05);总甲基化水平升高(P<0.05)。与B[a]P组比较,B[a]P+5-Aza-cdR组提高了海马神经元活性(P<0.05);降低细胞凋亡率(P<0.01);DNMTs基因和蛋白表达水平明显下降(P<0.05);总甲基化水平下降(P<0.01)。结论5-Aza-cdR对B[a]P诱导的海马神经元损伤有保护作用。Objective To probe the protective effects of 5-Aza-2’-deoxycytidine(5-Aza-cdR)on the neurotoxicity of benzo[a]pyrene(B[a]P)to hippocampal neurons.Methods The hippocampal neurons were cultured until the 5th day.The cells were observed and identified.The experiment was divided into control group,B[a]P group(20μmol/L,B[a]P),and B[a]P+5-Aza-cdR group(20μmol/L B[a]P+10μmol/L 5-Aza-cdR).Cell morphology was observed.Cell viability,DNA methyltransferase protein,and gene expression were detected.Total methylation level and apoptosis were measured.Results Compared with the control group,the B[a]P group decreased the viability of hippocampal neurons(P<0.05).The apoptosis rate increased(P<0.05).The expression level of DNMT3A,DNMT3B gene and DNMT1、DNMT3A protein increased(P<0.05).The total methylation level increased(P<0.05).Compared with the B[a]P group,the B[a]P+5-Aza-cdR group increased hippocampal neuronal viability(P<0.05),and the apoptosis rate decreased(P<0.01)and visibly decreased DNMTs gene and protein expression levels(P<0.05).The methylation level was obviously decreased(P<0.01).Conclusion 5-Aza-cdR has a protective effect on B[a]P-induced hippocampal neuron injury.

关 键 词：苯并[A]芘 5-氮杂-2’-脱氧胞苷 海马神经元 甲基化 

分 类 号：R734[医药卫生—肿瘤]