低氮胁迫下栽培大豆和野大豆幼苗适应性转录组学比较研究  

Comparative study on the adaptable transcriptomics of cultivated and wild soybean seedling under low nitrogen stress

作　　者：李明霞 周际 胡勇军 韩德复 郭继勋 李淑英 张涛 石连旋[3] LI Ming-xia;ZHOU Ji;HU Yong-jun;HAN De-fu;GUO Ji-xun;LI Shu-ying;ZHANG Tao;SHI Lian-xuan(School of Life Sciences,Changchun Normal University,Changchun 130032,China;Land Consolidation and Rehabilitation Centre,The Ministry of Land and Resources,Beijing 100035,China;School of Life Sciences,Northeast Normal University,Changchun 130024,China;Forestry and Grassland Bureau of Aohan Banner of Chifeng,Chifeng 024000,China)

机构地区：[1]长春师范大学生命科学学院,吉林长春130032 [2]自然资源部国土整治中心,北京100035 [3]东北师范大学生命科学学院,吉林长春130024 [4]内蒙古赤峰市敖汉旗林业和草原局,内蒙古赤峰024000

出　　处：《东北师大学报（自然科学版）》2023年第2期116-125,共10页Journal of Northeast Normal University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(32072012);吉林省自然科学基金资助项目(20200201134JC);长春师范大学自然科学基金资助项目(2021007)。

摘　　要：以栽培大豆和野大豆幼苗为实验材料,人工模拟低氮胁迫,采用转录组测序技术(RNA-seq)测试分析了胁迫处理下栽培大豆和野大豆幼苗在转录水平发生的变化,揭示了野大豆抵御低氮胁迫的分子机制.结果表明:野大豆能够通过促进根系生长维持较高的根冠比、增大根系与营养物质的接触面积吸收更多的氮抵御低氮胁迫.低氮胁迫下野大豆和栽培大豆幼苗叶片中分别有182个和733个差异表达基因,根系中分别有807个和621个差异表达基因.野大豆通过调控NRT1、NRT2和AMT蛋白家族相关基因的表达来抵御低氮胁迫.叶片中AP2/ERF-ERF、C2H2、C3H、GRAS、MYB、NAC、TIFY和WRKY转录因子,根系中C2H2、GRAS、MYB、NAC、TIFY和WRKY转录因子在野大豆抵御低氮胁迫的过程中起调控作用.野大豆抵御低氮胁迫的关键是增强叶片中的半胱氨酸、蛋氨酸代谢和谷胱甘肽代谢,增强根系中的半胱氨酸、蛋氨酸、氰基氨酸代谢,N-聚糖生物合成、氨基糖和核苷酸糖代谢及糖酵解.Using cultivated soybean and wild soybean seedling as experimental materials,artificially simulated low nitrogen stress,and used transcriptome sequencing(RNA-seq)test to analyze the changes in the transcription level of cultivated soybean and wild soybean seedling under stress treatment,revealing the molecular mechanism of wild soybean resisted low nitrogen stress.The results showed that wild soybean could resist low nitrogen stress by promoting root growth,maintaining a high root to shoot ratio,increasing the contact area between roots and nutrients,and absorbing more nitrogen.Transcriptomics studies showed that there were 182 and 733 differentially expressed genes in wild and cultivated soybean seedling leaves;807 and 621 differentially expressed genes in wild and cultivated soybean seedling roots under low nitrogen stress,respectively.Wild soybean resisted low nitrogen stress by regulating the expression of related genes of NRT1,NRT2 and AMT protein family.AP2/ERF-ERF,C2H2,C3H,GRAS,MYB,NAC,TIFY and WRKY transcription factors in leaves;the transcription factors C2H2,GRAS,MYB,NAC,TIFY and WRKY in roots played a regulatory role in the process of wild soybean resisted low nitrogen stress.The KEGG database enrichment analysis showed that the key to resist low nitrogen stress in wild soybean was to enhance the cysteine methionine metabolism and glutathione metabolism in leaves;enhance the cysteine methionine metabolism,cyano acid metabolism,N-glycan biosynthesis,amino sugar and nucleotide sugar metabolism and glycolysis in roots.

关 键 词：栽培大豆 野大豆 低氮胁迫 转录组学 

分 类 号：Q945.78[生物学—植物学]