蕨麻cpSSR序列特征分析  

Characterization Analysis of Microsatellite in Chloroplast of Potentilla anserina

作　　者：田甜 李军乔 王鑫慈 曲俊儒 TIAN Tian;LI Jun-qiao;WANG Xin-ci(The College of Ecological Environment and Resources,Qinghai Minzu University,Xining,Qinghai 810007;.2.Tibetan Plateau Juema Industry Research Institute,Xining,Qinghai 810007)

机构地区：[1]青海民族大学生态环境与资源学院,青海西宁810007 [2]青藏高原蕨麻产业研究院,青海西宁810007

出　　处：《安徽农业科学》2023年第12期94-97,共4页Journal of Anhui Agricultural Sciences

基　　金：青海省重点研发与转化计划项目(2022-NK-120);青海民族大学2021年研究生创新项目(954M2021005)。

摘　　要：基于NCBI数据库中收录的蕨麻叶绿体基因组序列,利用REPuter软件和MISA软件分析其微卫星分布规律。结果显示,在叶绿体基因组中共检测到34个散在重复序列,22个分布在LSC区,10个分布在IR区,2个分布在SSC区。使用MISA共注释到92个SSR位点,平均每1820 bp出现1个SSR位点。SSR主要分布在LSC区(79.07%),重复序列主要以单碱基为主,占总数量的86.05%。微卫星长度平均为12 bp,大多数长度为10 bp,说明蕨麻中重复基元较短的微卫星变异速率较快。The chloroplast genome sequences of Potentilla anserina L.was used to analyze the distribution of microsatellite from Gen Bank Database.It was analyzed with bioinformatics software REPuter and MISA.The results showed that it owned a total of 34 dispersed repetitive sequence in the chloroplast genome of Potentilla anserina L.,mainly distributed in the large single-copy region and Inverted repeat,22 and 10 respective,the small single-copy region merely 2.Using the MISA found 92 microsatellites in the chloroplast genome of Potentilla anserina L.One SSR locus per 1820 bp.Mainly distributed in the large single-copy region(79.07%),the most commom repeat type of the cpSSR is mononucleotide repeat which accountede for 86.05%.The average length of SSR is 12 bp,mainly concentrated in 10 bp,which suggested that the shorter length of SSR in Potentilla anserina L.is change faster.

关 键 词：蕨麻 叶绿体基因组 微卫星 

分 类 号：Q943[生物学—植物学]