原生质体再生技术复壮金针菇退化菌株的研究  

Study on rejuvenating degenerated strain of Flammulina filiformis by protoplast regeneration technology

作　　者：张丹[1] 朱秋瑾 谭琦[1] 宋春艳[1] 于海龙[1] 王瑞娟[1] 尚晓冬[1] 刘建雨 ZHANG Dan;ZHU Qiujin;TAN Qi;SONG Chunyan;YU Hailong;WANG Ruijuan;SHANG Xiaodong;LIU Jianyu(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,National Engineering Research Center of Edible Fungi,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China)

机构地区：[1]上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点开放实验室,上海201403

出　　处：《上海农业学报》2023年第3期10-16,共7页Acta Agriculturae Shanghai

基　　金：上海市科技兴农项目[沪农科创字(2020)第1-3号]。

摘　　要：利用原生质体再生技术对白色金针菇退化菌株进行复壮,研究复壮后菌株与正常菌株的菌丝生长性状及工厂化栽培的农艺性状,并对复壮前后的菌株进行了基于转录组的单核苷酸多态性检测和特异表达基因分析。结果表明:原生质体再生技术是白色金针菇菌种复壮的有效手段,复壮菌株的菌丝生长速率、生育期、原基形成时间、菇盖直径、菌柄长度、单瓶子实体数量以及单瓶产量可恢复至正常菌株水平。复壮菌株较退化菌株的遗传背景发生了变化,其单核苷酸多态性位点数比退化菌株多,且大部分位于基因密码子区域。复壮菌株中与细胞吞噬、能量代谢、抗氧化、细胞骨架构建以及细胞DNA甲基化修饰等相关的基因特异表达。The protoplast regeneration technology was used to rejuvenate the degenerated strain of Flammulina filiformis,and the mycelial growth traits and agronomic traits of industrial cultivation of the rejuvenated strain and the normal strain were studied.The single nucleotide polymorphism detection and specific expression gene analysis based on transcriptome were carried out for the strain before and after rejuvenation.The results showed that protoplast regeneration technology was an effective means for rejuvenation of F.filiformis.The mycelial growth rate,growth period,primordium formation time,pileus diameter,stipe length,fruiting body number per bottle and yield per bottle of rejuvenated strain could be restored to the level of normal strain.The genetic background of the rejuvenated strain had changed compared to the degraded strain,with an increase in the number of single nucleotide polymorphic loci,most of which were located in the gene codon region.The genes related to cell phagocytosis,energy metabolism,antioxidant,cytoskeleton construction,and cell DNA methylation modification were specifically expressed in rejuvenated strain.

关 键 词：金针菇 菌种退化 复壮 原生质体再生 单核苷酸多态性 

分 类 号：S646.1[农业科学—蔬菜学]