谱域光学相干层析成像定量表征皮肤癌组织结构  

Quantitative evaluation of morphology of skin cancer tissue by spectral domain optical coherence tomography

作　　者：杨志刚 刘兵[1] 王金滢 顾振宇 吴刘颖 陆原[2] 屈军乐[1] YANG Zhigang;LIU Bing;WANG Jinying;GU Zhenyu;WU Liuying;LU Yuan;QU Junle(College of Physics and Optoelectronic Engineering,Shenzhen University,Shenzhen 518060,Guangdong Province,P.R.China;Department of Dermatology,Huazhong University of Science and Technology Shenzhen Hospital,Shenzhen 518052,Guangdong Province,P.R.China)

机构地区：[1]深圳大学物理与光电工程学院,广东深圳518060 [2]华中科技大学协和深圳医院皮肤科,广东深圳518052

出　　处：《深圳大学学报（理工版）》2023年第4期401-406,共6页Journal of Shenzhen University(Science and Engineering)

基　　金：国家自然科学基金资助项目(61875131)。

摘　　要：皮肤癌等疾病的发生严重危害身体健康,为研究皮肤癌组织皮下结构变化与动态监测皮肤癌发生过程对皮肤结构的影响,采用谱域光学相干层析成像(spectral domain optical coherence tomography,SD-OCT)技术研究离体基底细胞癌皮肤组织,建立裸鼠背脊皮翼视窗(dorsal skin fold window chamber,DSFC)黑色素瘤模型.通过层析扫描对病变组织皮下结构进行图像重构,分别获得了皮肤样品一维扫描信号、二维层析图像和三维结构图像.对比分析发现,基底细胞癌皮肤外部形态无明显变化,内部组织存在无散射信号区域,二维层析图像中表现为椭圆形状黑色巢穴.黑色巢穴病灶区位于皮肤表皮下层区域,长度约155.972μm,宽度约150.192μm.同时,通过对DSFC黑色素瘤模型连续11 d的动态监测,发现伴随黑色素瘤细胞的分裂增殖,皮肤表面出现肿瘤状隆起,皮下毛细血管逐渐清晰.在二维OCT图像中,光学系统光束穿透能力受到限制,在肿瘤种植后不同时间节点位置的层析成像深度呈现衰减区域,而且皮肤层OCT信号增强,可能因为黑色素瘤的生长并不断向上侵袭所致.实验结果表明,离体基底细胞癌皮肤组织内部形态发生显著变化,出现无OCT信号黑色病灶区域;裸鼠在体黑色素瘤的动态发展对皮肤组织产生影响,不同的时间节点,皮肤层具有不同的OCT影像表现.该研究为基底细胞癌的诊断和治疗提供了图像和数据依据,同时动态监测黑色素瘤过程也对皮肤癌病变过程研究具有重要意义.The occurrences of skin cancers and malignant diseases jeopardize human health.To study the changes of subcutaneous structure of skin cancer tissue in vitro and monitor the dynamics of skin cancer development in skin structure,spectral domain optical coherence tomography(SD-OCT)was used to scan the skin tissue of basal cell carcinoma in vitro and to establish the model of dorsal skin fold window chamber(DSFC)melanoma in nude mouse.One dimensional scanning signal,two dimensional tomographic image and three dimensional structural image of the skin sample were obtained by tomographic scanning and imaging reconstruction of the subcutaneous structure of the lesion tissue.Comparative analysis showed no significant change presented in the external morphology of the skin of basal cell carcinoma.There was non-scattered signal area in the internal tissue.Two-dimensional tomographic images showed an elliptically black-nest lesion located in the subepidermal area of the skin with about 155.972μm in length and 150.192μm in width.Meanwhile,the DSFC melanoma with tomographic imaging model was consecutively observed for 11 days.It showed that tumor bulges appeared on the skin surface and subcutaneous capillaries became clear with the proliferation of melanoma cells.In the two dimensional OCT image,the penetrating depth of the laser beam decreased with the depth of tomography at different time after tumor implantation attenuates,but the OCT signal from the skin layer enhanced due to the strong scattering effect.It is probably caused by the generation of melanoma and its continuous invading upwards.The results show that the internal morphology of the skin tissue of basal cell carcinoma in vitro has changed,and black lesion region without any OCT signal appeared.The dynamic development of melanoma in nude mouse in vivo has an impact on skin tissue,varied morphological form in the skin layer at different time periods is revealed by OCT images.The study provides the promising support in image and data basis for the diagnosis and tr

关 键 词：肿瘤学 激光生物学 谱域光学相干层析成像 基底细胞癌 黑色素瘤 皮肤组织结构 背脊皮翼视窗 皮肤组织动态观察 

分 类 号：R739.5[医药卫生—肿瘤] Q631[医药卫生—临床医学]