敲低CASC9表达对食管鳞癌细胞增殖、迁移和侵袭的影响  

Effect of down-regulating CASC9 expression on proliferation,migration and invasion in esophageal squamous cell carcinoma

作　　者：李倩倩[1] 唐茂林 赵世圣 胡为民[1] LI Qian-qian;TANG Mao-lin;ZHAO Shi-sheng;HU Wei-min(Department of Immunology,School of Basic Medicine and Forensic Medicine,North Sichuan Medical College,Nanchong 637000,Sichuan,China)

机构地区：[1]川北医学院基础医学与法医学院免疫学教研室,四川南充637000

出　　处：《川北医学院学报》2023年第7期867-871,共5页Journal of North Sichuan Medical College

基　　金：四川省南充市科技局资助项目(19SXHZ0344);川北医学院科研发展基金项目(CBY15-A-YB18)。

摘　　要：目的:探讨lncRNA CASC9对食管鳞癌细胞增殖、迁移和侵袭及相关调控因子的影响。方法:采用RT-qPCR检测3种食管鳞癌细胞株中CASC9的表达情况。采用si-CASC9沉默Eca109、Kyse150细胞中CASC9的表达,采用RT-qPCR检测si-CASC9沉默效率;采用CCK-8检测NC组及si-CASC9组细胞的增殖活力;采用细胞划痕实验检测NC组及si-CASC9组的细胞迁移速度;采用Traswell侵袭实验检测NC组及si-CASC9组细胞侵袭能力;采用RT-qPCR和Western blot检测RhoA的mRNA及蛋白的表达情况。结果:CASC9在Eca109、TE-1和Kyse150 3种食管鳞癌细胞株中的表达水平比较,差异有统计学意义(P<0.05),Eca109和Kyse150中表达量较高;沉默CASC9表达后,Eca109、Kyse150细胞增殖、迁移和侵袭能力减弱(P<0.05)。沉默CASC9表达后,RhoA的mRNA和蛋白的表达均上调(P<0.05)。结论:沉默CASC9的表达,可抑制Eca109和Kyse150细胞的增殖和迁移和侵袭能力,可能与调节RhoA的表达相关。Objective:To investigate the effect of CASC9 on proliferation,migration and invasion in esophageal squamous cell carcinoma(ESCC).Methods:The CASC9 mRNA expression of three kinds of ESCC cell lines were detected by RT-qPCR.The expression of CASC9 was inhibited by si-CASC9 technology in Eca109 and Kyse150 cell-lines,silencing efficiency of si-CASC9 detected by RT-qPCR.The proliferation activity of NC group and si-CASC9 group was detected by CCK8.The cell migration ability of NC group and si-CASC9 group was detected by wound healing assay.The cell invasion ability of NC group and si-CASC9 group was detected by Transwell.RT-qPCR and Western blot were used to detect the mRNA and protein expression of RhoA.Results:The expression levels of CASC9 in three esophageal squamous cell carcinoma cell lines(Eca109,TE-1,and Kyse150)were statistically significant(P<0.05).Eca109 and Kyse150 had higher expression.After interfering the expression of CASC9,the proliferation,migration,and invasion abilities of Eca109 and Kyse150 cells decreased(P<0.05),the mRNA and protein expression of RhoA were upregulated(P<0.05).Conclusion:Interfering the expression of CASC9 can inhibit the proliferation,migration,and invasion ability of Eca109 and Kyse150 cells,which may be related to regulating the expression of RhoA.

关 键 词：食管鳞癌 CASC9 RHOA 增殖 迁移 

分 类 号：R735.1[医药卫生—肿瘤]