竹叶花椒黄酮类物质抗氧化活性及其谱效关系  

Spectrum-effect Relationship of Antioxidant Activity of Flavonoids from Zanthoxylum armatum DC.

作　　者：何鑫柱 李潮俊 周孟焦 康明[2] 梁晓峰 HE Xinzhu;LI Chaojun;ZHOU Mengjiao;KANG Ming;LIANG Xiaofeng(Laboratory of Research Development and Utilization on Traditional Chinese Medicine Resources in Northwest Sichuan,Sichuan College of Traditional Chinese Medicine,Mianyang,Sichuan 621010,China;School of Materials and Chemistry,Southwest University of Science and Technology,Mianyang,Sichuan 621010,China)

机构地区：[1]四川中医药高等专科学校川西北中药材资源研究与开发利用实验室,四川绵阳621010 [2]西南科技大学材料与化学学院,四川绵阳621010

出　　处：《农产品加工》2023年第12期54-57,共4页Farm Products Processing

基　　金：四川省中医药管理局科学技术研究专项课题(2020JC0120);绵阳市科技项目(2020YFZJ004)。

摘　　要：利用DPPH法和高效液相色谱法(High Performance Liquid Chromatography,HPLC)分别获得了10批次竹叶花椒果皮黄酮类物质的抗氧化活性和HPLC指纹图谱。运用偏最小二乘回归(Partial Least Squares Regression,PLSR)法分析HPLC指纹图谱共有峰峰面积与抗氧化活性IC50值之间的关系。结果表明,竹叶花椒果皮提取的黄酮类物质清除DPPH自由基的IC50值在14.530~20.833μg/g,其HPLC指纹图谱中可标定出16个共有峰,其中主要抗氧化活性成分是表儿茶素、金丝桃苷、紫云英苷和山奈酚。The antioxidant activity and HPLC fingerprints of 10 batches of flavonoids from the peel of Zanthoxylum armatum DC were obtained by DPPH method and high performance liquid chromatography.The partial least squares regression method was used to analyze the relationship between the common peak area of HPLC fingerprints and the IC50 value of antioxidant activity.The results showed that the IC50 values of the flavonoids extracted from the peel of Zanthoxylum armatum DC for scavenging DPPH free radicals were between 14.530 and 20.833μg/g,and 16 common peaks could be demarcated in the HPLC fingerprint,and the main antioxidant activity ingredients were epicatechin,hyperoside,astragaline and kaempferol.

关 键 词：竹叶花椒 黄酮 抗氧化活性 谱效关系 

分 类 号：TQ91[化学工程]