牛磺酸对胰腺癌细胞系BxPC-3和PANC-1细胞增殖、凋亡和迁移的影响  

Effects of taurine on proliferation,apoptosis and migration of human pancreatic cancer cell lines BxPC-3 and PANC-1

作　　者：高慧婕[1] 李倩 田斌 朱日明 刘超[1] GAO Huijie;LI Qian;TIAN Bin;ZHU Riming;LIU Chao(College of Pharmacy,Jining Medical University,Rizhao 276827,China;Pharmacy Department,Qingdao Women and Children's Hospital;Clinical Laboratory,People's Hospital of Rizhao)

机构地区：[1]济宁医学院药学院,276827 [2]青岛市妇女儿童医院药学部 [3]日照市人民医院检验科

出　　处：《天津医药》2023年第7期707-712,共6页Tianjin Medical Journal

基　　金：教育部产学合作协同育人项目(202101237026);济宁市重点研发计划项目(2022YXNS159)。

摘　　要：目的探讨牛磺酸(Tau)对胰腺癌导管细胞增殖、凋亡和迁移的影响。方法体外培养胰腺癌BxPC-3和PANC-1细胞,采用不同浓度Tau(0、10、20、40、80、160 mmol/L)进行预处理。采用CCK-8、细胞划痕实验、TUNEL法观察Tau对BxPC-3和PANC-1细胞增殖、迁移和凋亡的影响;实时荧光定量聚合酶链反应(qPCR)和蛋白质免疫印迹法检测Tau影响BxPC-3和PANC-1细胞中相关细胞凋亡及细胞周期分子mRNA和蛋白表达的情况。结果Tau可抑制BxPC-3和PANC-1的细胞增殖和迁移活性;同时,Tau能够促进BxPC-3和PANC-1细胞凋亡。与对照组相比,Tau处理后的BxPC-3细胞中相关凋亡因子P53、P21、Bcl-2关联X蛋白(BAX)表达水平呈上升趋势,而增殖细胞核抗原(PCNA)的表达降低;Tau处理后的PANC-1细胞中B淋巴细胞瘤-2蛋白(Bcl-2)、PCNA、细胞周期蛋白CyclinA2、CyclinB1、CyclinE、周期蛋白依赖性激酶CDK1、CDK2、CDK4、CDK6等的表达较对照组均下降,而相关凋亡蛋白P53、P21的表达水平升高。结论Tau可抑制胰腺癌细胞BxPC-3和PANC-1的增殖和迁移活性,并促进细胞凋亡,可能是通过影响相关细胞凋亡基因和细胞周期蛋白来抑制胰腺癌细胞的活性。Objective To investigate the effects of taurine(Tau)on the proliferation,apoptosis and migration of pancreatic cancer ductal cells.Methods BxPC-3 and PANC-1 cells were cultured in vitro and pretreated with different concentrations of Tau(0,10,20,40,80,160 mmol/L).Cell counting kit-8(CCK-8),cell scratch assay and Terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL)method were used to observe the effect of Tau on the proliferation,migration and apoptosis of BxPC-3 and PANC-1 cells.Quantitative real-time polymerase chain reaction(qPCR)and Western blot assay were used to detect the effect of Tau on mRNA and protein expression of relevant apoptosis and cell cycle molecules in BxPC-3 and PANC-1 cells.Results Tau significantly inhibited the proliferation and migration activities of BxPC-3 and PANC-1,and Tau could promote the apoptosis of BxPC-3 and PANC-1 cells.Compared with the control group,expression levels of P53,P21 and BAX in BxPC-3 cells treated with Tau showed a significant upward trend,while the expression of PCNA was significantly decreased.The expression levels of Bcl-2,PCNA,CyclinA2,CyclinB1,CyclinE,CDK1,CDK2,CDK4 and CDK6 in PANC-1 cells were significantly lower than those in the normal control group,while the expression levels of P53 and P21 showed an upward trend.Conclusion Tau can inhibit the proliferation and migration activity of BxPC-3 and PANC-1 pancreatic cancer cells,and promote cell apoptosis,possibly by affecting related apoptosis genes and cyclins.

关 键 词：牛磺酸 胰腺肿瘤 肿瘤抑制蛋白质P53 周期素依赖激酶抑制剂p21 细胞周期蛋白类 周期蛋白依赖性激酶 

分 类 号：R349.5[医药卫生—基础医学]