基于高通量测序的偏执型精神分裂症患者肠道菌群的差异性研究  

The differential research of gut microbiota in paranoid schizophrenia patients based on high-throughput sequencing

作　　者：高晓峰 何鲜艳 胡伟明 倪燕飞 于恩彦 Gao Xiaofeng

机构地区：[1]浙江省衢州市第三医院,324000 [2]浙江省肿瘤医院,310011

出　　处：《浙江临床医学》2023年第6期805-807,共3页Zhejiang Clinical Medical Journal

基　　金：浙江省医药卫生科技计划项目(2020KY1027);浙江省科技厅重点研发项目(2021C03106)。

摘　　要：目的探究偏执型精神分裂症患者肠道菌群的差异及与临床症状的相关性。方法以2019年2月至2020年4月收治的27例符合入选标准的偏执型精神分裂症患者设为观察组,选取同期31例符合入选标准的健康体检者设为对照组,收集两组患者清晨空腹状态下中段粪便标本,采用16SrDNA基因测序技术,比较两组肠道菌群的差异以及与临床症状的相关性。结果通过对两组肠道特定菌群水平进行统计,发现观察组体内柯林斯菌属、小杆菌属、脱硫弧菌属、嗜胆菌属、粪厌氧棒杆菌及毛螺菌属的丰度与对照组比较差异均具有统计学意义,其中柯林斯菌属、小杆菌属、脱硫弧菌属和嗜胆菌属表达值显著高于对照组(P<0.05);粪厌氧棒杆菌、毛螺菌属表达值显著低于对照组(P<0.05)。观察组的柯林斯菌属、粪厌氧棒杆菌、毛螺菌属、小杆菌属、脱硫弧菌属、嗜胆菌属与PANSS阳性分、阴性分、一般病理分及总分之间的相关性进行分析,发现嗜胆菌属和阳性分、一般病理分和总分存在正相关,差异具有统计学意义(P=0.045、P=0.043和P=0.035),说明阳性症状和一般精神症状与其可能存在一定关系。结论在偏执型精神分裂症患者与健康人中,肠道菌群的构成存在显著差异,并且与临床症状存在一定的相关性,可能为解析偏执型精神分裂症的发病机制提供新的思考。Objective To explore differences of gut microbiota in patients with paranoid schizophrenia and its correlation with clinical symptoms.Methods 27 patients with paranoid schizophrenia who met the inclusion criteria were selected as the research group,and 31 healthy subjects who met the inclusion criteria during the same period were selected as the control group from February 2019 to April 2020.16SrDNA gene sequencing technology was used to compare the differences in gut microbiota between the two groups,SPSS was used to show the correlation with clinical symptoms and gut microbiota.Results The abundance of Collinsella,Dialister,Desulfovibrio,Bilophila,Anaerostipes and Lachnospira were found the significant difference compared with the control group.Collinsella,Dialister,Desulfovibrio and Bilophila were significantly higher than those in the control group(P<0.05).Anaerostipes and Lachnospira were significantly lower than those of the control group(P<0.05).Correlation analysis was used to show relationship between the Collinsella,Anaerostipes,Lachnospira,Dialister,Desulfovibrio,Bilophila and PANSS scores in the study group.It was found that there was a positive correlation between Bilophila and positive score,general pathological scores and total score,and the difference was statistically significant(P=0.045,P=0.043 and P=0.035,respectively),indicating that positive symptoms and general mental symptoms might have a certain relationship.Conclusion There are significant differences in the composition of gut microbiota between paranoid schizophrenia patients and healthy people,and there is a certain correlation with clinical symptoms,which may provide a new point for developing the pathogenesis of paranoid schizophrenia.

关 键 词：高通量测序 偏执型精神分裂症 肠道菌群 

分 类 号：R749.3[医药卫生—神经病学与精神病学]