杭州市1株不产毒霍乱弧菌食品分离株的基因组特征与溯源研究  

Genomic characteristics and source tracing of a non-toxigenic Vibrio cholerae food isolate in Hangzhou

作　　者：俞骅[1] 高佳佳 朱炜 郑伟[1] 郑之北[1] 吴金飞 王毅意 潘高翔 汪皓秋[1] YU Hua;GAO Jiajia;ZHU Wei;ZHENG Wei;ZHENG Zhibei;WU Jinfei;WANG Yiyi;PAN Gaoxiang;WANG Haoqiu(Hangzhou Center for Disease Control and Prevention,Zhejiang Hangzhou 310021,China;Binjiang District Center for Disease Control and Prevention,Zhejiang Hangzhou 310051,China)

机构地区：[1]杭州市疾病预防控制中心,浙江杭州310021 [2]滨江区疾病预防控制中心,浙江杭州310051

出　　处：《中国食品卫生杂志》2023年第4期491-497,共7页Chinese Journal of Food Hygiene

基　　金：杭州市科技发展计划(20191203B141);杭州市医学重点学科建设项目资助。

摘　　要：目的研究杭州市1株不产毒小川血清型霍乱弧菌食品分离株与国内外食品和临床分离株的溯源关系及其分子特征。方法采用荧光定量聚合酶链式反应检测菌株毒力基因分布,使用高通量测序获得基因组序列,使用生物信息学方法鉴定其多位点序列分型(MLST)型别。将101株食品和临床分离株基因组序列比对到参考基因组N16961获得单核苷酸突变位点(SNV),去除重组并构建进化树,分析食品分离株和近源菌株的基因分布情况。结果该食品分离株是不产霍乱毒素和产毒素共调菌毛的霍乱弧菌(CNTP),MLST分型结果为ST1480,与杭州地区流行的ST75型CNTP菌株属于同一克隆群。该菌属于进化树L3b.1分支,与杭州2011年临床分离株(HZ11-1,HZ11-2)高度同源,去除重组后仅有26个SNVs位点差异。结论从甲鱼涂抹拭子中检出与杭州地区流行的CNTP高度同源的菌株,杭州地区散发病例感染来源可能与爬行类食品污染因素有关,应加强对其养殖场所及销售场所进行消毒。Objective To study the relationship and molecular characteristics of a non-toxic ogawa serotype Vibrio cholerae food isolate with food and clinical isolates at home and abroad.Methods Quantitative polymerase chain reaction(qPCR)and high-throughput sequencing was applied to obtain virulent genes distribution and genome sequence.Bioinformatic methods were used to identify their multi-locus sequencing typing(MLST)types.One hundred and one isolates genomes were mapped to reference genome Vibrio cholerae N16961 to obtain the single nucletiode variant(SNV).All alignment was applied to construct the evolutionary tree after removing recombination.Comparative genome analysis was applied to the genes presence and absence in food isolates and close strains.Results The food isolate was a cholera toxin negative and toxin-coregulated pilus positive(CNTP)strain of Vibrio cholerae.The MLST type was ST1480 which belonged to the same clonal complex of the ST75 CNTP strain prevalent in Hangzhou.It was in the branch of L3b.1 in the phylogenic tree,and highly homologous to clinical isolates in 2011(HZ11-1,HZ11-2).After removing the recombination,there were only 26 different SNVs.Conclusion The food isolate was highly close to the clones of the CNTP Vibrio cholerae L3b.1 branch in Hangzhou.The source of infection in the sporadic cases in Hangzhou may be related to the contamination of reptile so that the disinfection of its breeding sites and sales sites should be strengthen.

关 键 词：霍乱弧菌 霍乱毒素 多位点序列分型 基因组进化树 

分 类 号：R155[医药卫生—营养与食品卫生学]