miR-26a-5p调控SLC26A4挽救听力减退在耳聋中的作用  

Effect of MiR-26a-5p Regulating SLC26A4 to Ameliorate Hearing Loss in Deafness

作　　者：丛林海[1] 汤勇[1] 殷家志 邓苙[2] 高慧芳 方红丽 李昕[3] CONG Linhai;TANG Yong;YIN Jiazhi;DENG Li;GAO Huifang;FANG Hongli;LI Xin(Dept.of Otolaryngology,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032,China;Health Administration Center,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032,China;Clinical Laboratory,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032,China)

机构地区：[1]昆明医科大学第一附属医院耳鼻喉科,云南昆明650032 [2]昆明医科大学第一附属医院健康管理中心,云南昆明650032 [3]昆明医科大学第一附属医院检验科,云南昆明650032

出　　处：《昆明医科大学学报》2023年第6期14-18,共5页Journal of Kunming Medical University

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(202001AY070001-211)。

摘　　要：目的检测miR-26a-5p和SLC26A4在耳聋小鼠和听力损失小鼠中的表达变化,以此研究miR-26a-5p调控SLC26A4挽救听力减退的作用。方法通过构建小鼠听力损失模型和小鼠耳聋模型,使用听性脑干反应测试检测小鼠右耳的听力来鉴定模型的构建是否成功。使用qPCR实验检测miR-26a-5p和SLC26A4在小鼠听力损失和耳聋后的相对表达量,通过TargetScan网站预测miR-26a-5p靶向结合SLC26A4的具体位点,对小鼠注射miR-26a-5p和SLC26A4的干扰和过表达载体,继续检测miR-26a-5p和SLC26A4的表达变化。结果在小鼠听力损失和耳聋后,miR-26a-5p表达显著降低(P<0.05),而SLC26A4表达明显升高(P<0.05),在小鼠注射antagomir后,miR-26a-5p明显下调(P=0.047),同时SLC26A4显著上调(P=0.014),同时小鼠听力阈值明显降低(P<0.05),而在注射miR-26a-5p agomir后结果恰恰相反。结论miR-26a-5p可以通过SLC26A4从而降低听力减退以此抑制耳聋的发生。Objective To explore the effect of miR-26a-5p in regulating SLC26A4 to amiliorate hearing loss,and to detect the expression changes of miR-26a-5p and SLC26A4 in deaf mice and hearing loss mice.Methods A mouse hearing loss model and a mouse deafness model were constructed,and the auditory brainstem response test was used to detect the hearing in the right ear of mice to determine whether the models were successfully constructed.qPCR test was used to detect the relative expression of miR-26a-5p and SLC26A4 after hearing loss and deafness in mice were established.The specific site of miR-26a-5p targeting binding to SLC26A4 was predicted at TargetScan website.The interference and overexpression vector of miR-26a-5p and SLC26A4 were injected,and the expression changes of miR-26a-5p and SLC26A4 were detected.Results After hearing loss and deafness were established,the expression of miR-26a-5p was significantly decreased(P<0.05),while the expression of SLC26A4 was significantly increased(P<0.05).After antagomir injection,miR-26a-5p was significantly down-regulated(P=0.047),while SLC26A4 was significantly up-regulated(P=0.014);and the hearing threshold of mice decreased significantly(P<0.05),but the result was just the opposite after injection of miR-26a-5p agomir.Conclusion miR-26a-5p can ameliorate hearing loss through SLC26A4 and thus decelerate the development of deafness.

关 键 词：听力减退 耳聋 miR-26a-5p SLC26A4 

分 类 号：R764.431[医药卫生—耳鼻咽喉科]