B淋巴细胞瘤-2关联永生基因3蛋白、多重肿瘤抑制基因1在宫颈癌癌前病变中表达及联合液基细胞学检查的临床意义  

Expression of BAG3 protein and P16 in patients with cervical precancerous lesions and its clinical significance in combination with liquid based cytology

作　　者：杜晓琴 马瑛 谢刚 陈丽娟 潘长清 张勇 DU Xiaoqin;MA Ying;XIE Gang;CHEN Lijuan;PAN Changqing;ZHANG Yong(Department of Obstetrics and Gynecology,Mianyang Central Hospital Affiliated to University of Electronic Science and Technology of China,Mianyang,Sichuan 621000,China)

机构地区：[1]电子科技大学医学院附属绵阳医院·绵阳市中心医院妇产科,四川绵阳621000

出　　处：《安徽医药》2023年第8期1633-1636,共4页Anhui Medical and Pharmaceutical Journal

基　　金：绵阳市科技计划项目(16S-ZC-04)。

摘　　要：目的研究B淋巴细胞瘤-2关联永生基因3(BAG3)蛋白、多重肿瘤抑制基因1(P16)在宫颈癌癌前病变中表达及联合液基细胞学检查的临床意义。方法选择绵阳市中心医院2015年6月至2017年6月在妇科门诊筛查并确诊的120例宫颈病变病人为研究对象,根据其病变情况分为两组,其中癌前病变组病人69例,宫颈癌组病人51例。对比两组病人的BAG3、P16水平,将不同严重程度宫颈癌癌前病变病人的BAG3、P16水平进行比较,分析联合检测效能。结果宫颈癌组病人的BAG3(2.74±0.37)水平、P16(1.45±0.38)水平显著高于癌前病变组BAG3(1.70±0.51)、P16(0.53±0.16),差异有统计学意义(P<0.05)。不同严重程度宫颈癌癌前病变病人的BAG3和P16水平差异有统计学意义(P<0.05),其中宫颈上皮内瘤变Ⅰ级(CINⅠ)组的BAG3(1.01±0.24)水平低于CINⅡ组BAG3(1.78±0.79)和CINⅢ组的BAG3(2.33±0.88),且CINⅡ组低于CINⅢ组,差异有统计学意义(P<0.05);CINⅠ组的P16(0.21±0.06)水平低于CINⅡ组P16(0.45±0.10)和CINⅢ组的P16(0.72±0.17)水平,且CINⅡ组低于CINⅢ组,差异有统计学意义(P<0.05)。联合诊断对于宫颈癌的诊断特异度显著高于单独检测,差异有统计学意义(P<0.05)。通过ROC曲线分析结果发现联合检测对于宫颈癌癌前病变的诊断ROC曲线下面积显著高于单独检测(P<0.05)。结论随着宫颈癌前病变的进展,P16、BAG3表达增加,BAG3蛋白、P16联合液基细胞学检查对于病人的诊断具有积极的意义。Objective To study the expression of B lymphocytoma-2 associated immortality gene 3(BAG3)protein,multiple tumor suppressor gene 1(P16)in patients with cervical precancerous lesions and the clinical significance of combined liquid based cytology.Methods A total of 120 patients with cervical disease screened and diagnosed in the Gynecological Clinic of Mianyang Central Hospital from June 2015 to June 2017 were selected as the research objects.According to the pathological changes,they were divided into two groups,including 69 patients with precancerous lesions and 51 patients with cervical cancer.The levels of BAG3 and P16 in the two groups and patients with cervical precancerous lesions of different severity were compared,and the efficacy of combined detection,including levels of BAG3,P16 and liquid based cytology was analyzed.Results The levels of BAG3(2.74±0.37)and P16(1.45±0.38)in cervical cancer group were significantly higher than those in precancerous lesion group(1.70±0.51)and P16(0.53±0.16),with statistical significance(P<0.05).The difference of BAG3 and P16 levels in patients with cervical precancerous lesions of different severity was statistically significant(P<0.05).The level of BAG3 in CINⅠgroup(1.01±0.24)was lower than that in CINⅡgroup(1.78±0.79)and CINⅢgroup(2.33±0.88),and CINⅡgroup was lower than CINⅢgroup,the difference was statistically significant(P<0.05).The level of P16 in CINⅠgroup(0.21±0.06)was lower than that in CINⅡgroup(0.45±0.10)and CINⅢgroup(0.72±0.17),and CINⅡgroup was lower than CINⅢgroup,the difference was statistically significant(P<0.05).The specificity of combined diagnosis for cervical cancer was significantly higher than that of single detection(P<0.05).ROC curve analysis showed that the area under the ROC curve of combined detection was significantly higher than that of single detection(P<0.05).Conclusion With the progression of cervical precancerous lesions,the expression of p16 and BAG3 increases,and the combination of BAG3 protein and P16 combi

关 键 词：宫颈肿瘤 组织细胞学制备技术 癌前病变 B淋巴细胞瘤-2关联永生基因3(BAG3) 联合检测 多重肿瘤抑制基因1(P16) 

分 类 号：R737.33[医药卫生—肿瘤]