Nanosilver alleviates foreign body reaction and facilitates wound repair by regulating macrophage polarization  

作　　者：Chuangang YOU Zhikang ZHU Shuangshuang WANG Xingang WANG Chunmao HAN Huawei SHAO 

机构地区：[1]Department of Burns&Wound Care Center,the Second Afiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310009,China [2]Department of Burns,the First People's Hospital of Wenling,Wenling 317500,China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2023年第6期510-523,共14页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：supported by the Zhejiang Province Key Research and Development Program(No.2019C03083);the Zhejiang Provincial Basic Public Welfare Research Program(No.LGF19H150008);the National Natural Science Foundation of China(Nos.81601681 and 81871558).

摘　　要：Foreign body reactions induced by macrophages often cause delay or failure of wound healing in the application of tissue engineering scaffolds.This study explores the application of nanosilver(NAg)to reduce foreign body reactions during scaffold transplantation.An NAg hybrid collagen-chitosan scaffold(NAg-CCS)was prepared using the freeze-drying method.The NAg-CCS was implanted on the back of rats to evaluate the effects on foreign body reactions.Skin tissue samples were collected for histological and immunological evaluation at variable intervals.Miniature pigs were used to assess the effects of NAg on skin wound healing.The wounds were photographed,and tissue samples were collected for molecular biological analysis at different time points post-transplantation.NAg-CCS has a porous structure and the results showed that it could release NAg constantly for two weeks.The NAg-CCS group rarely developed a foreign body reaction,while the blank-CCS group showed granulomas or necrosis in the subcutaneous grafting experiment.Both matrix metalloproteinase-1(MMP-1)and tissue inhibitor of metalloproteinase-1(TIMP-1)were reduced significantly in the NAg-CCS group.The NAg-CCS group had higher interleukin(IL)-10 and lower IL-6 than the blank CCS group.In the wound healing study,M1 macrophage activation and inflammatory-related proteins inducible nitric oxide synthase(iNOS),IL-6,and interferon-(IFN-)were inhibited by NAg.In contrast,M2 macrophage activation and proinflammatory proteins(arginase-1),major histocompatibility complex-II(MHC-II),and found in inflammatory zone-1(FIZZ-1)were promoted,and this was responsible for suppressing the foreign body responses and accelerating wound healing.In conclusion,dermal scaffolds containing NAg suppressed the foreign body reaction by regulating macrophages and the expression of inflammatory cytokines,thereby promoting wound healing.

关 键 词：NANOSILVER MACROPHAGES IMPLANTS Foreign body reaction Wound repair 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]