茶树FtsH基因家族鉴定及CsFtsH31功能分析  

Identification of FtsH gene family and functional analysis of CsFtsH31 gene

作　　者：何仕玉 姚新转 刘洋 陈凌霄 吕立堂[1,2] HE Shiyu;YAO Xinzhuan;LIU Yang;CHEN Lingxiao;LYU Litang(College of Life Sciences,Guizhou University,Guiyang,Guizhou 550025,China;College of Tea Science,Guizhou University,Guiyang,Guizhou 550025,China;College of Tobacco Science,Guizhou University,Guiyang,Guizhou 550025,China)

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州大学茶学院,贵州贵阳550025 [3]贵州大学烟草学院,贵州贵阳550025

出　　处：《福建农业学报》2023年第5期583-597,共15页Fujian Journal of Agricultural Sciences

基　　金：贵州省科技计划项目(黔科合支撑[2021]一般111)。

摘　　要：【目的】FtsH基因在植物的抗逆境胁迫中具有重要的作用,揭示茶树FtsH基因在茶树中的功能和表达模式可为茶树耐光氧化和抗高温胁迫改良育种提供理论依据。【方法】通过茶树基因组数据鉴定FtsH基因,对其进行生物信息学分析,筛选出1个对高温敏感的CsFtsH31基因。在烟草中超量表达CsFtsH31基因,通过光氧化和高温处理分析CsFtsH31基因的表达模式。【结果】在茶树基因组中共鉴定到了45个FtsH基因,按照进化关系可将其分成5类,拟南芥和茶树FtsH基因同源性很高。该家族成员分布在茶树不同的染色体上。茶树中CsFtsH基因在不同组织和不同胁迫条件下存在差异表达,在高温处理后茶树中CsFtsH14、CsFtsH31、CsFtsH34基因随着时间变化呈上升趋势,CsFtsH31对高温尤其敏感;遗传转化CsFtsH31基因获得转CsFtsH31基因烟草,对转CsFtsH31基因烟草进行光氧化和高温胁迫处理,结果发现转基因烟草的叶绿素、可溶性糖含量及超氧化物歧化酶活性均高于野生型,而丙二醛含量低于野生型。【结论】CsFtsH31基因在烟草中表达,提高叶绿素、可溶性糖含量及超氧化物歧化酶活性,降低丙二醛,清除胁迫产生的活性氧,保护膜结构功能,提高烟草的抗光氧化和抗高温胁迫能力。【Objective】FtsH gene plays an important role in plant stress resistance.Revealing the function and expression pattern of FtsH gene in tea plant can provide a theoretical basis for the improvement of tea plant resistance to photooxidation and high temperature stress.【Method】The FtsH gene was identified by tea plant genome data,and a CsFtsH31 gene sensitive to high temperature was screened by bioinformatics analysis.The CsFtsH31 gene was overexpressed in tobacco,and the expression patterns of CsFtsH31 gene against photooxidation and high temperature were analyzed.【Result】A total of 45 FtsH genes were identified in the genome of tea plant,which were divided into five categories according to the evolutionary relationship.The members of this family are distributed on different chromosomes of tea plants.CsFtsH genes in tea plants are differentially expressed in different tissues and stress conditions.After high temperature treatment,CsFtsH14,CsFtsH31 and CsFtsH34 genes in tea plants showed an increasing trend over time,and CsFtsH31 is particularly sensitive to high temperature.The transgenic tobacco with CsFtsH31 gene was obtained by genetic transformation of CsFtsH31 gene.The transgenic tobacco with CsFtsH31 gene was subjected to stress treatment.The results showed that the chlorophyll and soluble sugar content and superoxide dismutase activity of transgenic tobacco were higher than wild type,while malondialdehyde was lower than wild type.【Conclusion】The CsFtsH31 gene was expressed in tobacco by increasing the content of chlorophyll and soluble sugar,and the activity of superoxide dismutase,reducing the content of malondialdehyde,scavenging the reactive oxygen species produced by stress,protecting the function of membrane structure,and improving the resistance of tobacco to light oxidation and high temperature stress.

关 键 词：茶树 烟草 CsFtsH基因 转CsFtsH31基因烟草 抗光氧化 抗高温 

分 类 号：S571.1[农业科学—茶叶生产加工]