运动和2,3,7,8-四氯二苯并二噁英(TCDD)持续暴露对大鼠肾脏氧化应激指标的影响  

Effects of Exercise and Continuous Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD)on Renal Oxidative Stress Indexes in Rats

作　　者：朱凤林 闫会萍 陆一帆 胡兵 张弛 翁凯翔 王活活 Zhu Fenglin;Yan Huiping;Lu Yifan;Hu Bing;Zhang Chi;Weng Kaixiang;Wang Huohuo(.National Institute of Sports Medicine,General Administration of Sport of China,Beijing 100029,China;School of Sport Medicine and Rehabilitation,Beijing Sport University,Beijing 100084,China)

机构地区：[1]国家体育总局运动医学研究所,北京100029 [2]北京体育大学运动医学与康复学院,北京100084

出　　处：《生态毒理学报》2023年第2期366-372,共7页Asian Journal of Ecotoxicology

基　　金：中央高校基本科研业务费(2017YB026);国家重点研发计划项目(2018YFC2000603)。

摘　　要：观察8周有氧运动和2,3,7,8-四氯二苯并二噁英(2,3,7,8-tetrachlorodibenzo-p-dioxin,2,3,7,8-TCDD)持续暴露对大鼠肾脏组织氧化应激指标及相关蛋白水平的影响。选取40只8周龄雄性Sprague-Dawley(SD)大鼠,随机分为空白对照组(NC组)、运动组(EC组)、TCDD暴露组(NT组)和运动+TCDD暴露组(ET组),NT组和ET组进行8周TCDD处理(腹腔注射,每周1次)首次剂量为6.4μg·kg^(-1),第2~8周采用维持剂量(为首次剂量的21%),EC组和ET组采用游泳运动干预方式(5%体质量的尾部负重,每周5次)。8周干预后,测定血清尿素氮(urea nitrogen,BUN)、肌酐(creatinine,Cr),肾脏氧化应激相关指标脂质过氧化水平、谷胱甘肽(glutathione,GSH)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)以及细胞色素P4501A1(cytochrome P4501A1,CYP1A1)蛋白含量。结果显示,运动可降低大鼠体质量和肾脏质量,提高GSH-Px活性;TCDD暴露可降低血清BUN水平,提高脂质过氧化水平、GSH含量、GSH-Px和SOD活性,提高CYP1A1蛋白含量;TCDD暴露下运动可提高GSH-Px活性,降低CYP1A1蛋白含量。本研究表明,TCDD暴露可导致大鼠肾脏发生氧化应激,可能与TCDD诱导的CYP1A1增加有关;8周中等强度有氧运动未能明显缓解TCDD诱导的氧化应激,但能显著降低TCDD诱导的CYP1A1水平,具体机制有待进一步研究。This study aims to investigate the renal oxidative stress indexes and related protein levels in response to exercise and 2,3,7,8-tetrachlorodibenzo-p-dioxin(2,3,7,8-TCDD)exposure.Forty 8-week-old male Sprague-Dawley(SD)rats were randomly divided into blank control group(NC group),exercise group(EC group),TCDD exposure group(NT group)and exercise+TCDD exposure group(ET group).Both NT and ET groups were treated with TCDD for 8 weeks(intraperitoneal injection,weekly).The initial dose was 6.4μg·kg^(-1),followed by a maintenance dose(21%of the initial dose)from week 2 to 8.Exercise intervention were prescribed for both EC and ET groups in the form of swimming(tail loading at 5%of body weight,five times per week).After 8 weeks of intervention,serum urea nitrogen(BUN)and creatinine(Cr),indicators related to renal oxidative stress including lipid peroxidation level,glutathione(GSH),glutathione peroxidase(GSH-PX),superoxide dismutase(SOD),catalase(CAT)and cytochrome P4501A1(CYP1A1)protein relative content were measured.The results showed that exercise could reduce body weight and kidney weight,and increase GSH-Px activity.TCDD exposure resulted serum BUN reduction,and increased lipid peroxidation level,GSH content,GSH-Px and SOD activity,and CYP1A1 level.The combination of exercise intervention and TCDD exposure can increase GSH-Px activity and decrease CYP1A1 level.This study indicated that TCDD exposure can cause oxidative stress in rat kidneys,which may be related to the TCDD-induced increase of CYP1A1.Moderate-intensity aerobic exercise for 8 weeks could not significantly relieve TCDD-induced oxidative stress,but could significantly reduce the level of TCDD-induced CYP1A1.The specific mechanism needs further research.

关 键 词：2 3 7 8-四氯二苯并二噁英 大鼠 氧化应激 运动 

分 类 号：X171.5[环境科学与工程—环境科学]