不同浓度石油胁迫处理下翅碱蓬的转录组分析  

Transcriptome Analysis of Suaeda heteroptera under Different Concentrations of Oil Stress

作　　者：赵雨朦 卢宏博 罗传辉 车鉴 吴熙 纪峰 霍玉洁 魏海峰 何洁 刘全 Zhao Yumeng;Lu Hongbo;Luo Chuanhui;Che Jian;Wu Xi;Ji Feng;Huo Yujie;W ei Haifeng;He Jie;Liu Quan(Technology Innovation Center for Coastal Ecological Environment and Disaster Protection,Liaoning Province,Dalian 116023,China;Key Laboratory of Nearshore Marine Environmental Science and Technology in Liaoning Province,Dalian 116023,China;School of Marine Technology and Environment,Dalian Ocean University,Dalian 116023,China;Dalian Xinyulong Marine Biological Seed Technology Co.Ltd.,Dalian 116222,China;College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China)

机构地区：[1]辽宁省近海生态环境与灾害防护工程技术创新中心,大连116023 [2]辽宁省近海岸海洋环境科学与技术重点实验室,大连116023 [3]大连海洋大学海洋科技与环境学院,大连116023 [4]大连鑫玉龙海洋生物种业科技股份有限公司,大连116222 [5]大连海洋大学水产与生命学院,大连116023

出　　处：《生态毒理学报》2023年第2期490-505,共16页Asian Journal of Ecotoxicology

基　　金：大连市科技重大专项“辽参绿色生态养殖关键技术研发与应用”(2020ZD23SN009);国家重点研发计划“海洋环境安全保障”重点专项(2019YFC1407700);国家海洋公益性行业科研专项(201305002);辽宁省教育厅服务地方项目(DL201804);国家海洋局海洋溢油鉴别与损害评估技术重点实验室开放基金(201309,201809)。

摘　　要：为探究翅碱蓬响应石油胁迫的分子机制,本试验设置5个处理组:空白对照组、丙酮对照组、低浓度胁迫组(0.4 mg·L^(-1))、中浓度胁迫组(0.8 mg·L^(-1))和高浓度胁迫组(1.2 mg·L^(-1)),通过水培方式将翅碱蓬植株培养12 d后,对植株进行全转录组测序。结果表明,与空白对照组(A)进行对比,丙酮对照组(B)共获得1027个差异表达基因,其中上调基因448个,下调基因579个;与丙酮对照组进行对比,低浓度胁迫组(C)共得到522个差异表达基因,中浓度胁迫组(D)共得到1027个差异表达基因,高浓度胁迫组(E)共得到632个差异表达基因。对KEGG富集通路以Q≤0.05为标准进行进一步筛选发现,翅碱蓬响应丙酮胁迫主要涉及植物病原相互作用、植物激素信号转导和MAPK信号通路;响应石油胁迫下毒性效应的途径主要涉及乙醛酸和二羧酸代谢、碳代谢和精氨酸、脯氨酸代谢。综上,石油胁迫不利于翅碱蓬的生长代谢,其中丙酮作为石油助溶剂对植物也产生了负面影响。同时植物也通过自身机制应对胁迫,包括去除活性氧(ROS)、调节激素水平和调节相关氨基酸含量等。试验结果可望为深入研究翅碱蓬响应石油胁迫的分子机制提供数据参考。To explore the molecular mechanism of Suaeda heteroptera in response to petroleum stress,five treatment groups were set up in this experiment:control group,acetone control group,low concentration group(0.4 mg·L^(-1)),medium concentration group(0.8 mg·L^(-1)),and high concentration group(1.2 mg·L^(-1)).After 12 d of hydroponics,the plants were subjected to whole-transcriptome sequencing.Experimental results show that compared with the blank control group(A),a total of 1027 differentially expressed genes were obtained in the acetone control group(B),among them,448 were increased and 579 were decreased.Compared with the acetone control group,522 differentially expressed genes were obtained in the low concentration group(C),1027 differentially expressed genes were obtained in the medium concentration group(D),632 differentially expressed genes were obtained in the high concentration group(E).Further screening of the KEGG pathway with Q≤0.05 as the standard found that the response to acetone stress by Suaeda heteroptera mainly involves plant-pathogen interaction,plant hormone signal transduction and MAPK signaling pathway;the response to petroleum stress mainly involves glyoxylate and dicarboxylate metabolism,carbon metabolism and arginine and proline metabolism pathway.In summary,petroleum stress is detrimental to the growth and metabolism of winged alkali ponies,in which acetone as a petroleum co-solvent also has a negative impact on the plants.Meanwhile,plants also responded to petroleum stress through their own mechanisms,including removal of reactive oxygen species(ROS),regulation of hormone levels,and regulation of relevant amino acid content.This study is expected to provide data reference for further research on the molecular mechanism of Suaeda heteroptera in response to petroleum stress.

关 键 词：石油胁迫 翅碱蓬 代谢通路 转录组测序 

分 类 号：X171.5[环境科学与工程—环境科学]